两种用于检测2C-B及相关致幻苯乙胺的免疫分析法。

Two immunoassays for the detection of 2C-B and related hallucinogenic phenethylamines.

作者信息

Šuláková Anna, Fojtíková Lucie, Holubová Barbora, Bártová Kateřina, Lapčík Oldřich, Kuchař Martin

机构信息

Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Technická 5, 166 28 Praha 6, Dejvice, Czechia; Forensic Laboratory of Biologically Active Substances, University of Chemistry and Technology Prague, Technická 3, 166 28 Praha 6, Dejvice, Czechia; Department of Experimental Neurobiology, National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czechia.

Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Technická 5, 166 28 Praha 6, Dejvice, Czechia; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 3, 166 28 Praha 6, Dejvice, Czechia.

出版信息

J Pharmacol Toxicol Methods. 2019 Jan-Feb;95:36-46. doi: 10.1016/j.vascn.2018.11.001. Epub 2018 Nov 24.

DOI:10.1016/j.vascn.2018.11.001

PMID:30481558

Abstract

INTRODUCTION

The use of new psychoactive substances as drugs of abuse has dramatically increased over the last years. Hallucinogenic phenethylamines gained particular popularity as they have both stimulating and psychedelic effects. Although generally perceived as safe, these illicit drugs pose a serious health risk; they have been linked to cases of severe poisoning or even deaths. Therefore, simple, cost-effective and reliable methods are needed for rapid determination of abused hallucinogens.

METHODS

For this purpose, two haptens derived from 2C-H were designed, synthesized and subsequently attached to a carrier protein. Polyclonal antibodies obtained from a rabbit immunized with one of the prepared immunogens were used for the development of two immunoassays.

RESULTS

In this study, a lateral flow immunoassay (LFIA) and an enzyme linked immunosorbent assay (ELISA) for the detection of 2C-B and related hallucinogenic phenethylamines in urine were developed. The presented LFIA is primarily suitable for on-site monitoring as it is simple and can provide a visual evidence of 2C-B presence within a few minutes. Its reasonable sensitivity (LOD = 15 ± 7 ng mL) allows detection of the drug presence in urine after acute exposure. For greater accuracy, highly sensitive ELISA (LOD = 6 ± 3 pg mL) is proposed for toxicological quantitative analyses of positive samples captured by the LFIA.

DISCUSSION

The comparison of the ELISA with the well-established UHPLC-MS-MS method shows excellent agreement of results, which confirms good potential of the ELISA to be used for routine analyses of 2C-B and related hallucinogenic phenethylamines of both main sub-families.

摘要

引言

在过去几年中，新型精神活性物质作为滥用药物的使用量急剧增加。致幻苯乙胺因其具有刺激和迷幻作用而特别受欢迎。尽管通常被认为是安全的，但这些非法药物却构成严重的健康风险；它们与严重中毒甚至死亡病例有关。因此，需要简单、经济高效且可靠的方法来快速测定滥用的致幻剂。

方法

为此，设计、合成了两种源自2C-H的半抗原，随后将其连接到载体蛋白上。用其中一种制备的免疫原免疫兔子获得的多克隆抗体用于开发两种免疫测定法。

结果

在本研究中，开发了一种用于检测尿液中2C-B和相关致幻苯乙胺的侧向流动免疫测定法（LFIA）和酶联免疫吸附测定法（ELISA）。所展示的LFIA主要适用于现场监测，因为它操作简单，能在几分钟内提供2C-B存在的直观证据。其合理的灵敏度（检测限=15±7 ng/mL）能够检测急性暴露后尿液中药物的存在。为了获得更高的准确性，建议使用高灵敏度的ELISA（检测限=6±3 pg/mL）对LFIA捕获的阳性样品进行毒理学定量分析。