Petit M C, Mahuzier G
CHS Villejuif, Laboratoire de Biologie.
Ann Biol Clin (Paris). 1988;46(5):301-11.
Recent progress in biochemistry has shown the occurrence of many important peptides, among antibiotics, immunostimulants, hormones and neuromediators. The mass spectrometric study of these components by classical ionization techniques such as electron impact, chemical ionization or field desorption require a prior chemical derivatization because of their amphoteric properties, their low volatility and low thermostability. Recent improvements in methods of ionization, fast atom bombardment, secondary ion mass spectrometry, or mass spectrometry/mass spectrometry have made it possible to study the structure of peptides with more than 15 amino acid residues and eased mass spectra interpretation. Through the different methodologies described in this review three characteristic informations can be obtained concerning the molecular mass of peptides, the nature of constituent amino acids and the peptidic sequence and even quantitative measurements can be performed.
生物化学领域的最新进展表明，在抗生素、免疫刺激剂、激素和神经介质中存在许多重要的肽。由于这些肽具有两性性质、低挥发性和低热稳定性，采用经典电离技术（如电子轰击、化学电离或场解吸）对其进行质谱研究需要事先进行化学衍生化。电离方法（如快原子轰击、二次离子质谱或串联质谱）的最新进展使得研究含有15个以上氨基酸残基的肽的结构成为可能，并简化了质谱解析。通过本综述中描述的不同方法，可以获得有关肽的分子量、组成氨基酸的性质和肽序列的三个特征信息，甚至还可以进行定量测量。
