Tongtawe P, Chaicumpa W, Tapchaisri P, Looareesuwan S, Webster H K
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1988 Jun;6(1):11-8.
Lymphocytes of normal healthy persons were separated from blood by Ficoll-Hypaque gradient centrifugation and iron-magnet application. peripheral blood lymphocytes (PBL) were stained by various dye-labeled monoclonal antibodies. Cells positive for specific surface markers were enumerated by a fluorescence activated cell sorter (FACS) and fluorescence microscope (FM). The results revealed that the percentages of cells positive with one monoclonal antibody counted by these two techniques were similar while the percentages of cells with double staining were higher when counted by FACS than by FM. Lymphocyte subpopulations of 18 patients infected with Plasmodium falciparum during acute and convalescence period were studied. Lymphocytopenia occurred during the acute infection while total white blood cell counts were normal. PBL of the patients were stained with OKT3, OKT4, OKT8, Leu-11 and a combination of Leu-7, Leu-1 monoclonal antibodies. The absolute numbers of all lymphocyte subpopulations were decreased during the acute infection while T8 positive cells were decreased in both percentage and absolute number. Thus T4:T8 ratio (1.7:1) became higher than normal (1.3:1) at this period. During convalescence phase, absolute numbers and percentages of Leu-7+, Leu-1+ and perhaps Leu-7+, Leu-11- cells which had low NK cell activity were significantly higher than during acute illness. The finding might explain why the NK cell activity was low during the convalescence period.
通过Ficoll-泛影葡胺梯度离心法和应用铁磁体从血液中分离出正常健康人的淋巴细胞。用各种染料标记的单克隆抗体对外周血淋巴细胞（PBL）进行染色。通过荧光激活细胞分选仪（FACS）和荧光显微镜（FM）对具有特定表面标志物的阳性细胞进行计数。结果显示，用这两种技术计数的一种单克隆抗体阳性细胞的百分比相似，而双色染色细胞的百分比通过FACS计数时高于FM计数。研究了18例急性和恢复期感染恶性疟原虫患者的淋巴细胞亚群。急性感染期间出现淋巴细胞减少，而白细胞总数正常。用OKT3、OKT4、OKT8、Leu-11以及Leu-7、Leu-1单克隆抗体组合对患者的PBL进行染色。急性感染期间所有淋巴细胞亚群的绝对数量均减少，而T8阳性细胞的百分比和绝对数量均减少。因此，此时T4:T8比值（1.7:1）高于正常水平（1.3:1）。在恢复期，NK细胞活性较低的Leu-7+、Leu-1+以及可能Leu-7+、Leu-11-细胞的绝对数量和百分比显著高于急性发病期。这一发现可能解释了恢复期NK细胞活性较低的原因。
