基于液相色谱-电喷雾电离质谱法对印度大豆（大豆（L.）Merr.）中异黄酮的表征。
LC-ESI-MS based characterisation of isoflavones in soybean ( (L.) Merr.) from India.
作者信息
Akitha Devi M K, Sravan Kumar S, Giridhar P
机构信息
Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka 570020 India.
出版信息
J Food Sci Technol. 2018 Dec;55(12):5045-5054. doi: 10.1007/s13197-018-3443-0. Epub 2018 Sep 29.
A total of twenty-one soybean varieties were screened for their morphological characteristics followed by isoflavone content analysis by HPLC. The total isoflavone (TI) content was found within a wide range of 140.9-1048.6 μg/g of soy in different varieties. The highest isoflavone content was found in MAUS-2 followed by DS_2613 and lowest in Karunae (140.9 μg/g of soy). Various isoflavone forms were identified by LC-ESI MS. Significant differences in the isoflavone content were observed for all the aglycones and their glucoside conjugates as well as total daidzein, total genistein, total glycitein, and TI. A positive correlation between TI content and growth stages was found during the progression of seed development. An increase of 5.4-fold and 5.3-fold of TI concentration was observed for JS 335 and MAUS-2 respectively, from early to green mature (R5-R8) stage of bean development.
共筛选了21个大豆品种的形态特征，随后通过高效液相色谱法分析异黄酮含量。不同品种大豆中的总异黄酮（TI）含量在140.9 - 1048.6μg/g的广泛范围内。异黄酮含量最高的是MAUS - 2，其次是DS_2613，最低的是Karunae（140.9μg/g大豆）。通过液相色谱 - 电喷雾质谱法鉴定了各种异黄酮形式。观察到所有苷元及其糖苷共轭物以及总黄豆苷元、总染料木黄酮、总大豆黄素和TI的异黄酮含量存在显著差异。在种子发育过程中，发现TI含量与生长阶段呈正相关。从豆类发育的早期到绿熟期（R5 - R8），JS 335和MAUS - 2的TI浓度分别增加了5.4倍和5.3倍。
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