使用多位点序列分型法对菌株进行分析。
Analysis of strains using multilocus sequence typing.
作者信息
Sharma Anshul, Kaur Jasmine, Lee Sulhee, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 South Korea.
出版信息
Food Sci Biotechnol. 2018 Jun 14;27(6):1755-1760. doi: 10.1007/s10068-018-0417-y. eCollection 2018 Dec.
The objective of this study was to perform genetic diversity analysis of 13 strains isolated from South Korean foods by multilocus sequence typing (MLST). For typing, seven housekeeping loci (, , , , , , and ) were selected, amplified and analyzed. Fifty-one polymorphic sites varying from 1 to 22 in each species were identified. Thirteen sequence types were generated with allele numbers ranged from 2 to 10. The overall relationship between strains was assessed by unweighted pair group method with arithmetic mean dendrogram and minimum spanning tree. In addition, combined spits tree analysis revealed intragenic recombination. No clear relationship was observed between the isolation sources and strains. The developed MLST scheme enhanced our knowledge of the population diversity of strains and will be used further for the selection of industrially important strain.
本研究的目的是通过多位点序列分型（MLST）对从韩国食品中分离出的13株菌株进行遗传多样性分析。为了进行分型，选择了7个管家基因座（、、、、、和），进行扩增和分析。在每个物种中鉴定出51个多态性位点，其数量从1到22不等。产生了13种序列类型，等位基因数范围为2至10。通过算术平均树状图和最小生成树的非加权配对组方法评估菌株之间的总体关系。此外，合并分裂树分析揭示了基因内重组。在分离源和菌株之间未观察到明确的关系。所开发的MLST方案增强了我们对菌株群体多样性的了解，并将进一步用于工业上重要菌株的选择。
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