蓝藻中藻蓝蛋白的分析级纯化
Analytical Grade Purification of Phycocyanin from Cyanobacteria.
作者信息
Khazi Mahammed Ilyas, Demirel Zeliha, Liaqat Fakhra, Dalay Meltem Conk
机构信息
Department of Biotechnology, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey.
Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey.
出版信息
Methods Mol Biol. 2020;1980:173-179. doi: 10.1007/7651_2018_202.
Phycocyanin is a blue-colored pigment-protein complex that exhibits numerous biofunctions such as anti-inflammation, antioxidation, antitumor, neuroprotective effect, and immunological enhancement. Purified phycocyanin has pharmaceutical and nutraceutical applications. In addition, as a nontoxic and non-carcinogenic natural coloring agent, phycocyanin has many applications in the food and cosmetic industries. This chapter describes a protocol for extraction and analytical grade purification of phycocyanin from cyanobacteria. The purification steps include (1) extraction of phycocyanin from biomass, (2) ammonium sulfate precipitation of phycocyanin and dialysis, and (3) purification of phycocyanin by gel filtration and ion-exchange chromatography.
藻蓝蛋白是一种蓝色色素 - 蛋白质复合物，具有多种生物功能，如抗炎、抗氧化、抗肿瘤、神经保护作用和免疫增强作用。纯化的藻蓝蛋白具有医药和营养保健应用。此外，作为一种无毒且无致癌性的天然着色剂，藻蓝蛋白在食品和化妆品行业有许多应用。本章描述了一种从蓝细菌中提取和分析级纯化藻蓝蛋白的方法。纯化步骤包括：（1）从生物质中提取藻蓝蛋白；（2）藻蓝蛋白的硫酸铵沉淀和透析；（3）通过凝胶过滤和离子交换色谱法纯化藻蓝蛋白。
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