Figueira Rebeca Lopes, Costa Karina Miura da, Marsico Ana Laura, Milani Thamires Melchiades da Silva, Gonçalves Walusa Assad, Borges Marcos de Carvalho, Silva Orlando Castro E, Sbragia Lourenço
Post Doctoral degree, Postgraduate Laboratory of Experimental Fetal Surgery, Division of Pediatric Surgery, Department of Surgery and Anatomy, Ribeirao Preto Medical School, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Scientific and intellectual content of the study; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; statistical analysis; manuscript preparation and writing.
Fellow PhD degree, Postgraduate Laboratory of Experimental Fetal Surgery, Division of Pediatric Surgery, Department of Surgery and Anatomy, Ribeirao Preto Medical School, USP, Ribeirao Preto-SP, Brazil. Technical procedures, manuscript writing, critical revision.
Acta Cir Bras. 2018 Oct;33(10):879-888. doi: 10.1590/s0102-865020180100000002.
To evaluate the vascular ventilatory response in different stages of lung development and to compare them to the neonates with congenital diaphragmatic hernia (CDH) in a rabbit model.
New Zealand rabbits were divided into 8 groups (n=5): E25, E27, E30, and CDH. All groups were ventilated on a FlexiVent (Scireq, Montreal, QC, Canada), compounding the other 4 groups. The CDH surgery was performed at E25 and the harvest at E30. Dynamic compliance (CRS), dynamic elastance (ERS) and dynamic resistance (RRS) were measured every 4 min/24 min. Median wall thickness (MWT) and airspace were measured. ANOVA Bonferroni tests were used to perform statistical analysis. Significance was considered when p<0.05.
CRS was higher in E30 compared to all other groups (p<0.05). CRS and RRS of CDH and E27 were similar and were higher in E25 (p<0.05). MWT was decreased according to the gestational age, was increased in E27V and E30V (p<0.05) and decreased in CDHV (p<0.05), airspace was decreased in E25 and increased in all ventilated groups (p<0.05).
The ventilation response of congenital diaphragmatic hernia is like the pseudoglandular stage of the lung development. These findings add information about the physiology of pulmonary ventilation in CDH.
在兔模型中评估肺发育不同阶段的血管通气反应，并将其与先天性膈疝（CDH）新生儿进行比较。
将新西兰兔分为8组（n = 5）：E25、E27、E30和CDH组。所有组均在FlexiVent（Scireq，加拿大魁北克省蒙特利尔）上进行通气，另外4组合并。CDH手术在E25进行，E30时进行取材。每4分钟/24分钟测量动态顺应性（CRS）、动态弹性（ERS）和动态阻力（RRS）。测量中膜厚度（MWT）和肺泡腔。采用方差分析Bonferroni检验进行统计分析。当p<0.05时认为具有显著性。
与所有其他组相比，E30组的CRS更高（p<0.05）。CDH组和E27组的CRS和RRS相似，E25组更高（p<0.05）。MWT随胎龄降低，E27V和E30V组增加（p<0.05），CDHV组降低（p<0.05），E25组肺泡腔减小，所有通气组肺泡腔增大（p<0.05）。
先天性膈疝的通气反应类似于肺发育的假腺期。这些发现增加了有关CDH肺通气生理学的信息。
