Division of Otolaryngology, Department of Ophthalmology, Otolaryngology and Head and Neck, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Division of Pediatric Surgery and Anatomy, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Clinics (Sao Paulo). 2024 Nov 5;79:100525. doi: 10.1016/j.clinsp.2024.100525. eCollection 2024.
The authors hypothesized that ventilation and hyperoxia may harm the cochlea vasculature in an experimental model of Congenital Diaphragmatic Hernia (CDH) performed in rabbits.
New Zealand rabbits underwent CDH creation at 25 days of gestation (term = 30 days). CDH was created in fetuses (n = 15) and compared with Controls (n = 15). Six groups were studied: Control, Control Ventilated 21% FiO (Control 21%), Control Ventilated 100% FiO (Control 100%), CDH, CDH Ventilated 21% FiO (CDH 21%) and CDH Ventilated 100% FiO (CDH 100%). Dynamic Compliance (CRS), dynamic Elastance (ERS), and dynamic Resistance (RRS) were measured. The cochleae were then removed, and the apical, middle, and basal slopes of the cochleae were evaluated. Samples were graded using a scoring system for the severity of bleeding: 0 (absent), 1 (mild), 2 (moderate), and 3 (severe). Statistical analysis was performed by contingence and ANOVA.
There was no difference in the severity of cochlear bleeding between Controls and CDH without ventilation. Control 21% and 100% had average scores of 2 and 1.2 respectively; CDH 21% and 100% had average scores of 0.4 and 3.8 respectively; RR [CDH 100% / Control 100%] (95% CI) = 3.16 (p < 0.005).
The severity of bleeding was 3.16 times worse with 100% oxygenation in CDH. This information may be helpful for future therapeutic strategies for decreasing SNHL in CDH patients.
作者假设，在兔先天性膈疝（CDH）模型中，通气和高氧可能会损害耳蜗血管。
25 日龄胎兔行 CDH 造模（足月=30 天）。CDH 造模胎儿（n=15）与对照（n=15）比较。共研究 6 组：对照组、21%FiO 通气对照组（Control 21%）、100%FiO 通气对照组（Control 100%）、CDH 组、21%FiO 通气 CDH 组（CDH 21%）和 100%FiO 通气 CDH 组（CDH 100%）。测量动态顺应性（CRS）、动态弹性（ERS）和动态阻力（RRS）。然后取出耳蜗，评估耳蜗的顶、中、底斜率。使用出血严重程度评分系统对耳蜗标本进行分级：0（无）、1（轻度）、2（中度）和 3（重度）。通过列联表和方差分析进行统计学分析。
未通气的 CDH 与对照组的耳蜗出血严重程度无差异。Control 21%和 100%的平均评分分别为 2 和 1.2；CDH 21%和 100%的平均评分分别为 0.4 和 3.8；RR [CDH 100%/Control 100%]（95%CI）=3.16（p<0.005）。
CDH 中 100%氧合时出血严重程度增加 3.16 倍。这些信息可能有助于未来制定 CDH 患者减少 SNHL 的治疗策略。
