大鼠睾丸精子发生衰老效应的体视学和形态学分析1

Stereological and morphological analysis of the effects of aging on spermatogenesis in rat testis 1.

作者信息

Miranda Eduardo Pereira de, Lorenzini Fernando, Neves Bruno Vinicius Duarte, Melchioretto Eduardo Felippe, Hota Thiago, Fraga Rogerio De

机构信息

Fellow Master degree, Postgraduate Program in Surgical Clinic, Department of Urology, Universidade Federal do Paraná (UFPR), Curitiba-PR, Brazil. Scientific, intellectual, conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; statistical analysis; manuscript preparation; critical revision.

PhD, Department of Urology, School of Medicine, UFPR, Curitiba-PR, Brazil. Scientific, intellectual, conception and design of the study; critical revision.

出版信息

Acta Cir Bras. 2018 Oct;33(10):904-913. doi: 10.1590/s0102-865020180100000005.

DOI:10.1590/s0102-865020180100000005

PMID:30484500

Abstract

PURPOSE

To quantify, through stereological and morphometric analysis, spermatogenesis in rats undergoing the natural aging process.

METHODS

Seventy-two male Wistar rats were divided into 6 equal groups according to age at the time of killing: 3, 6, 9, 12, 18, and 24 months. All the rats were subjected orchiectomy and collection of testicular parenchymal fragments for histological and morphometric analysis. The numerical density of spermatids was calculated using a stereological study, and morphometric analysis was conducted to measure the height of the germinal epithelium and the area of the seminiferous tubules.

RESULTS

We found that the 18 and 24 months groups showed a significant reduction in the number of round spermatids. However, the height of the germinal epithelium was not significantly different between the groups. The area of seminiferous tubules was also significantly reduced in the elderly rats compared to that in the young ones.

CONCLUSION

Aging of rats showed a significant reduction in the number of round spermatids and the area of the seminiferous tubules, more pronounced in the rats at 18 and 24 months of life.

摘要