Dušková M, Kolátorová L, Stárka L
Institute of Endocrinology, Prague, Czech Republic.
Physiol Res. 2018 Nov 28;67(Suppl 3):S379-S390. doi: 10.33549/physiolres.933964.
The androgens dehydroepiandrosterone sulfate, dehydro-epiandrosterone, androstenedione and testosterone are routinely assessed in women, and circulating levels of these androgens reflect their production. These androgens are measured in most laboratories using various immuno-analytical methods. Recently, however, androgen assays have begun to be performed using gas or liquid chromatography combined with mass spectrometry. To better understand the difficulties and issues of androgen laboratory diagnostics, it is important to assess each of the methods used, how and why they were introduced into practice, and their advantages, limits, historic milestones and current status. It is also necessary to understand how reference ranges are determined and specifics arising from the physiology of individual androgens. Here we present a summary and discussion of these issues.
女性通常会对硫酸脱氢表雄酮、脱氢表雄酮、雄烯二酮和睾酮等雄激素进行常规评估，这些雄激素的循环水平反映了它们的生成情况。大多数实验室使用各种免疫分析方法来检测这些雄激素。然而，近年来，雄激素检测已开始采用气相或液相色谱与质谱联用的方法。为了更好地理解雄激素实验室诊断的困难和问题，评估所使用的每种方法、它们如何以及为何被引入实际应用、其优点、局限性、历史里程碑和现状非常重要。还需要了解参考范围是如何确定的以及各个雄激素生理学所产生的具体情况。在此，我们对这些问题进行总结和讨论。
