Yeral Ilkin, Sayan Cemile D, Karaca Gökhan, Simsek Yavuz, Sagsoz Nevin, Ozkan Zehra S, Atasoy Pınar, Sahin Yasar, Neselioglu Salim, Erel Ozcan
Department of Obstetrics and Gynecology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey.
Department of General Surgery, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey.
J Obstet Gynaecol Res. 2019 Mar;45(3):592-599. doi: 10.1111/jog.13876. Epub 2018 Nov 28.
In this study, we aimed to investigate the protective effect of krill oil (KO) against ischemia-reperfusion (I/R) injury on rat ovary.
This study was conducted with 32 Wistar Albino rats. Rats were divided into four groups, with eight rats in each group-as follows: Sham group, I/R group, I/R + low dose KO group (50 mg) and I/R + high dose KO group (500 mg). The histopathological and follicle counts were performed on the right ovary. The total antioxidant status, total oxidant status and oxidative stress index were evaluated on the left ovary. And also serum N-thiol level, serum T-thiol level, serum disulfide (SDS) level, serum disulfide/N-thiol and serum disulfide/T-thiol ratios were evaluated too.
A statistically significant difference was determined between the I/R group and all the other groups for all parameters. There was significant difference between KO groups and the Sham group for the parameters of serum N-thiol, serum T-thiol, SDS, serum disulfide/N-thiol and serum disulfide/T-thiol. SDS, total oxidant status and oxidative stress index were determined to be the highest in the I/R group and the lowest in the low dose KO group. The total antioxidant status values were found to be the highest in the high dose KO group and the lowest in the I/R group. Follicle counts and histological injury scores showed no significant difference between Sham and KO groups.
This study demonstrated that KO has beneficial effects on decreasing the injury after I/R on rat ovary.
在本研究中，我们旨在探讨磷虾油（KO）对大鼠卵巢缺血再灌注（I/R）损伤的保护作用。
本研究使用32只Wistar白化大鼠进行。大鼠被分为四组，每组八只，分组如下：假手术组、I/R组、I/R +低剂量KO组（50毫克）和I/R +高剂量KO组（500毫克）。对右侧卵巢进行组织病理学检查和卵泡计数。对左侧卵巢评估总抗氧化状态、总氧化状态和氧化应激指数。同时还评估了血清N-硫醇水平、血清T-硫醇水平、血清二硫化物（SDS）水平、血清二硫化物/N-硫醇和血清二硫化物/T-硫醇比值。
对于所有参数，I/R组与所有其他组之间存在统计学上的显著差异。KO组与假手术组在血清N-硫醇、血清T-硫醇、SDS、血清二硫化物/N-硫醇和血清二硫化物/T-硫醇参数方面存在显著差异。SDS、总氧化状态和氧化应激指数在I/R组中最高，在低剂量KO组中最低。总抗氧化状态值在高剂量KO组中最高，在I/R组中最低。卵泡计数和组织学损伤评分在假手术组和KO组之间无显著差异。
本研究表明，KO对减轻大鼠卵巢I/R后的损伤具有有益作用。
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