Dayangan Sayan Cemile, Karaca Gokhan, Sema Ozkan Zehra, Tulmac Ozlem B, Ceylan Isik Aslı, Devrim Tuba, Aydin Gülcin, Yeral Ilkin
Department of Obstetrics and Gynecology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey.
Department of General Surgery, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey.
J Obstet Gynaecol Res. 2018 Feb;44(2):278-285. doi: 10.1111/jog.13524. Epub 2017 Nov 16.
The aim of this study was to investigate the protective effect of metformin on the rat ovary against ischemia-reperfusion injury.
Thirty-seven female Wistar albino rats were used in the study. The rats were divided into five groups, as follows: sham operation group (group 1); torsion group (group 2); torsion/detorsion + saline group (group 3); torsion/detorsion + low-dose metformin group (group 4); and torsion/detorsion + high-dose metformin group (group 5). The right ovary from each rat was evaluated histologically using hematoxylin-eosin staining, and the left ovaries were evaluated for tissue levels of the reduced-glutathione-to-oxidized-glutathione ratio, malondialdehyde (MDA), and caspase-3 activation.
The highest damage score was observed in group 3, and the lowest score was observed in group 1. The tissue caspase-3 activity levels of groups 2, 3, and 4 were significantly higher than those of group 1. The difference between group 1 and group 5 in terms of tissue caspase-3 activity was not significant (P = 0.4). The reduced-glutathione-to-oxidized-glutathione ratio of group 1 was significantly higher than the ratios found in groups 2, 3, and 4. The tissue MDA level of group 1 was significantly lower than the levels found in groups 2, 3, 4, and 5. The tissue MDA level of group 5 was significantly lower than the levels in groups 3 and 4.
From both histopathological and biochemical analyses, the results of the study demonstrated that metformin has beneficial effects when it comes to attenuating ovarian ischemia-reperfusion injury.
本研究旨在探讨二甲双胍对大鼠卵巢缺血再灌注损伤的保护作用。
本研究使用了37只雌性Wistar白化大鼠。大鼠被分为五组，如下：假手术组（第1组）；扭转组（第2组）；扭转/复位+生理盐水组（第3组）；扭转/复位+低剂量二甲双胍组（第4组）；扭转/复位+高剂量二甲双胍组（第5组）。使用苏木精-伊红染色对每只大鼠的右侧卵巢进行组织学评估，并对左侧卵巢的还原型谷胱甘肽与氧化型谷胱甘肽比值、丙二醛（MDA）水平和半胱天冬酶-3激活情况进行评估。
第3组观察到最高损伤评分，第1组观察到最低评分。第2、3和4组的组织半胱天冬酶-3活性水平显著高于第1组。第1组和第5组在组织半胱天冬酶-3活性方面的差异不显著（P = 0.4）。第1组的还原型谷胱甘肽与氧化型谷胱甘肽比值显著高于第2、3和4组。第1组的组织MDA水平显著低于第2、3、4和5组。第5组的组织MDA水平显著低于第3和4组。
从组织病理学和生化分析来看，本研究结果表明二甲双胍在减轻卵巢缺血再灌注损伤方面具有有益作用。
