Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Henan Jonint International Research Laboratory of Lymphoma, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
J Cell Mol Med. 2019 Feb;23(2):1509-1516. doi: 10.1111/jcmm.14057. Epub 2018 Nov 28.
Novel nude mice model of human NK/T cell lymphoma were established by subcutaneously injecting two NK/T cell lymphoma cell lines into the right axillary region of mice and successful passages were completed by injecting cell suspension which was obtained through a 70-μm cell strainer. These mice models and corresponding cell clones have been successfully developed for more than 8 generations. The survival rates of both resuscitation and transplantation in NKYS and YT models were 90% and 70% correspondingly. Pathologically, the tumour cells in all passages of the lymphoma-bearing mice and cell lines obtained from tumours were parallel to initial cell lines. Immunologically, the tumour cells expressed the characteristics of the primary and essential NK/T lymphomas. The novel mice models maintained the essential features of human NK/T cell lymphoma, and they would be ideal tools in vivo for further research of human NK/T cell lymphoma.
建立了人 NK/T 细胞淋巴瘤裸鼠模型，将两种 NK/T 细胞淋巴瘤细胞系皮下注射到裸鼠右侧腋窝，通过 70μm 细胞筛获得细胞悬液，成功传代 8 代以上。这些小鼠模型和相应的细胞克隆已成功开发超过 8 代。NKYS 和 YT 模型的复苏和移植存活率分别为 90%和 70%。从肿瘤中获得的肿瘤细胞和细胞系在所有传代中均与初始细胞系平行。免疫上，肿瘤细胞表达了原发性和基本 NK/T 淋巴瘤的特征。新型小鼠模型保持了人 NK/T 细胞淋巴瘤的基本特征，它们将成为人类 NK/T 细胞淋巴瘤体内研究的理想工具。
