Hesari AmirReza, Azizian Mitra, Darabi Hassan, Nesaei Abolfazl, Hosseini Seyede Atefe, Salarinia Reza, Motaghi Amir Ali, Ghasemi Faezeh
Department of Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
J Cell Biochem. 2019 May;120(5):7109-7114. doi: 10.1002/jcb.27984. Epub 2018 Nov 28.
One of the most lethal cancers among women is breast cancer. MicroRNAs (miRNAs) can be of great importance in the early detection of breast cancer. This study aimed to investigate some miRNAs in the serum of patients with breast cancer compared with the control group. Total RNA was extracted from the serum of patients with breast cancer and healthy volunteers. The expression levels of miRNAs and the genes were assessed using real-time reverse transcriptase-polymerase chain reaction with specific primers. Our data showed that miR-25 and miR-133 were downregulated, and miR-17 was upregulated in patients with breast cancer. Upregulation of miR-17 is related to the poor survival time and increased cell proliferation. The reduced expression of miR-133 and miR-25 is significantly associated with clinical stage, metastasis, and survival time of patients with breast cancer. Expressions of miRNAs miR-17, miR-25, and miR-133 are altered in patients with clinical stage, metastasis, poor survival time.
乳腺癌是女性中最致命的癌症之一。微小RNA（miRNA）在乳腺癌的早期检测中可能具有重要意义。本研究旨在调查乳腺癌患者血清中的一些miRNA，并与对照组进行比较。从乳腺癌患者血清和健康志愿者血清中提取总RNA。使用特异性引物通过实时逆转录-聚合酶链反应评估miRNA和基因的表达水平。我们的数据显示，乳腺癌患者中miR-25和miR-133表达下调，miR-17表达上调。miR-17的上调与较差的生存时间和细胞增殖增加有关。miR-133和miR-25表达降低与乳腺癌患者的临床分期、转移和生存时间显著相关。临床分期、转移、生存时间较差的患者中miR-17、miR-25和miR-133的表达发生改变。
