Han Jinghong, Wu Hanwei, Hu Hongyi, Yang Weiqiang, Dong Hongsong, Liu Yuchen, Nie Guohui
Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, China.
Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Cell Physiol Biochem. 2018;51(3):1437-1447. doi: 10.1159/000495592. Epub 2018 Nov 28.
BACKGROUND/AIMS: Hearing and balance deficits are mainly caused by loss of sensory inner ear hair cells. The key signals that control hair cell regeneration are of great interest. However, the molecular events by which the cellular signals mediate hair cell regeneration in the mouse utricle are largely unknown.
In the present study, we investigated gene expression changes and related molecular pathways using RNA-seq and qRT-PCR in the newborn mouse utricle in response to neomycin-induced damage.
There were 302 and 624 genes that were found to be up-regulated and down-regulated in neomycin-treated samples. GO and KEGG pathway analyses of these genes revealed many deregulated cellular components, molecular functions, biological processes and signaling pathways that may be related to hair cell development. More importantly, the differentially expressed genes included 9 transcription factors from the zf-C2H2 family, and eight of them were consistently down-regulated during hair cell damage and subsequent regeneration.
Our results provide a valuable source for future studies and highlighted some promising genes, pathways or processes that may be useful for therapeutic applications.
背景/目的：听力和平衡功能障碍主要由感觉性内耳毛细胞丢失引起。控制毛细胞再生的关键信号备受关注。然而，细胞信号介导小鼠椭圆囊毛细胞再生的分子事件在很大程度上尚不清楚。
在本研究中，我们使用RNA测序和定量逆转录聚合酶链反应，研究新生小鼠椭圆囊在新霉素诱导损伤后的基因表达变化及相关分子途径。
在新霉素处理的样本中，分别发现302个基因上调和624个基因下调。对这些基因的基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析揭示了许多失调的细胞成分、分子功能、生物学过程和信号通路，它们可能与毛细胞发育有关。更重要的是，差异表达基因包括9个zf-C2H2家族的转录因子，其中8个在毛细胞损伤及随后的再生过程中持续下调。
我们的结果为未来研究提供了有价值的资源，并突出了一些可能对治疗应用有用的有前景的基因、通路或过程。
