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比较两种用于鉴定海鞘组织中共生微生物群落特征的常见DNA提取试剂盒。

Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue.

作者信息

Evans James S, López-Legentil Susanna, Erwin Patrick M

机构信息

Department of Biology & Marine Biology, and Center for Marine Science, University of North Carolina Wilmington.

出版信息

Microbes Environ. 2018 Dec 28;33(4):435-439. doi: 10.1264/jsme2.ME18031. Epub 2018 Nov 27.

DOI:10.1264/jsme2.ME18031
PMID:30487350
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6308000/
Abstract

Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. Significant differences were detected in microbial community diversity and structure between ascidian species, but not between kits, suggesting similar recovery of biological variation and low technical variation between the two extraction methods for ascidian microbiome characterization.

摘要

尽管有迹象表明不同的技术会产生不同的结果，但各种DNA提取方法经常被交替使用来表征微生物群落。本文使用两种常见的DNA提取试剂盒，即DNeasy Blood和Tissue Kit（Qiagen）以及PowerSoil DNA Isolation Kit（Mo Bio Laboratories）对两种海鞘物种的微生物群进行了表征，随后对部分16S rRNA基因进行了二代（Illumina）测序。在海鞘物种之间检测到微生物群落多样性和结构存在显著差异，但在试剂盒之间未检测到显著差异，这表明两种提取方法在表征海鞘微生物群时对生物变异的回收率相似且技术变异较低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6274/6308000/2f791e209e3c/33_435_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6274/6308000/157ecc563428/33_435_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6274/6308000/2f791e209e3c/33_435_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6274/6308000/157ecc563428/33_435_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6274/6308000/2f791e209e3c/33_435_2.jpg

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