疱疹病毒感染与白细胞介素10之间的相互作用及骨髓抑制风险
Interaction between Herpes Virus Infections and IL10 and Risk of Bone Marrow Suppression.
作者信息
Yaghobi R, Alizadeh F, Khodavandi A
机构信息
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran.
出版信息
Int J Organ Transplant Med. 2018;9(3):119-125. Epub 2018 Aug 1.
BACKGROUND
Syndrome of transient bone marrow suppression may result from various extra-hematological diseases, such as immunological deregulations, and viral infectious diseases secondarily affecting the function of hematopoietic stem cells.
OBJECTIVE
To evaluate the pathogenic role of herpes viruses and their contraction with cytokine gene polymorphism, which can impair hematopoiesis in patients with transient bone marrow suppression.
METHODS
In a cross-sectional study 30 patients who admitted to Namazi Hospital, affiliated to Shiraz University of Medical Sciences, with transient bone marrow suppression were recruited. Diagnosis of the transient bone marrow suppression was made by expert hematologists. A control group consisting of 100 healthy unrelated individuals was also included. One EDTA-treated blood sample was collected from each studied patients and plasma was isolated. The molecular prevalence of cytomegalovirus and HHV8 evaluated was evaluated using real-time and nested PCR protocols, respectively. The SNPs of the (rs 1800896-1082G/A) cytokine gene was evaluated by PCR-RFLP method.
RESULTS
Cytomegalovirus and HHV8 infections were found in 2 and 3 of studied patients with transient bone marrow suppression. Significant higher frequency of G allele and GG genotype were found in HHV8-infected patients comparing to uninfected ones. Higher frequencies of A allele and AG and AA genotypes of were found in cytomegalovirus-uninfected patients comparing to infected ones, respectively. The significant higher frequencies of AA and AG genotypes were found in controls compared to bone marrow suppressed patients.
CONCLUSION
genetic polymorphism might have determinative role in resistance to the cytomegalovirus, especially HHV8 infections, in patients with bone marrow suppression. Focus in new interaction between HHV8 infection and genetics in bone marrow suppressed patients should be completed by the analysis of the anti-herpes virus immunity in future studies.
背景
短暂性骨髓抑制综合征可能由多种血液外疾病引起，如免疫失调以及继发影响造血干细胞功能的病毒感染性疾病。
目的
评估疱疹病毒的致病作用及其与细胞因子基因多态性的关联，细胞因子基因多态性可能损害短暂性骨髓抑制患者的造血功能。
方法
在一项横断面研究中，招募了设拉子医科大学附属纳马齐医院收治的30例短暂性骨髓抑制患者。短暂性骨髓抑制的诊断由血液学专家做出。还纳入了一个由100名健康无关个体组成的对照组。从每位研究患者采集一份乙二胺四乙酸（EDTA）处理的血样并分离血浆。分别使用实时荧光定量PCR和巢式PCR方法评估巨细胞病毒和人疱疹病毒8型（HHV8）的分子流行率。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估细胞因子基因（rs 1800896 - 1082G/A）的单核苷酸多态性（SNP）。
结果
在研究的短暂性骨髓抑制患者中，发现2例感染巨细胞病毒，3例感染HHV8。与未感染患者相比，HHV8感染患者中G等位基因和GG基因型的频率显著更高。与感染巨细胞病毒的患者相比，未感染巨细胞病毒的患者中A等位基因以及AG和AA基因型的频率分别更高。与骨髓抑制患者相比，对照组中AA和AG基因型的频率显著更高。
结论
基因多态性可能在骨髓抑制患者对巨细胞病毒尤其是HHV8感染的抵抗力中起决定性作用。未来研究应通过分析抗疱疹病毒免疫来完善对骨髓抑制患者中HHV8感染与基因之间新相互作用的关注。
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