Mishchenko T A, Mitroshina E V, Shishkina T V, Vedunova M B
N. I. Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia.
Bull Exp Biol Med. 2018 Dec;166(2):293-296. doi: 10.1007/s10517-018-4335-z. Epub 2018 Nov 28.
We propose an in vitro model of chronic oxidative stress based on the use of glucose oxidase. Oxidative stress modeling leads to a significant increase in the number of dead cells in culture. It was shown that the glial cell-derived neurotrophic factor exhibits a pronounced anti-oxidant effect. Preventive application of 1 ng/ml glial cell-derived neurotrophic factor significantly reduced the percentage of dead cells in culture.
我们提出了一种基于葡萄糖氧化酶使用的慢性氧化应激体外模型。氧化应激建模导致培养物中死细胞数量显著增加。结果表明，胶质细胞源性神经营养因子具有明显的抗氧化作用。预防性应用1 ng/ml胶质细胞源性神经营养因子可显著降低培养物中死细胞的百分比。
