微流控电泳中的样品预浓缩方案

Sample Preconcentration Protocols in Microfluidic Electrophoresis.

作者信息

Kitagawa Fumihiko, Otsuka Koji

机构信息

Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University, Hirosaki, Japan.

Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.

出版信息

Methods Mol Biol. 2019;1906:65-78. doi: 10.1007/978-1-4939-8964-5_4.

DOI:10.1007/978-1-4939-8964-5_4

PMID:30488385

Abstract

Electrophoretic on-line sample preconcentration techniques in microfluidic channels improve the sensitivity prior to the separation. Among various techniques, the most important field-amplified sample stacking and sweeping on cross-channel microchips are demonstrated. As a novel microfluidic preconcentration approach, a large-volume sample stacking with electroosmotic flow pump (LVSEP) on straight-channel chips is also presented, which can omit a complicated voltage program for sample injection processes. In this chapter, we describe how to prepare and how to run these on-line sample preconcentration methods in microchip electrophoresis.

摘要

微流控通道中的电泳在线样品预富集技术可在分离前提高灵敏度。在各种技术中，展示了最重要的场放大样品堆积和跨通道微芯片上的扫集。作为一种新型的微流控预富集方法，还介绍了在直通道芯片上利用电渗流泵进行的大体积样品堆积（LVSEP），它可以省略样品进样过程中复杂的电压程序。在本章中，我们描述了如何在微芯片电泳中制备和运行这些在线样品预富集方法。