Segasothy M, Kincaid-Smith P, Birch D F, Fairley K F
Department of Medicine, University of Melbourne, Victoria, Australia.
Clin Lab Med. 1988 Sep;8(3):483-92.
Accurate identification of nucleated cells in urine can be difficult with conventional methods of microscopic urinalysis. Monoclonal antibodies were used with an immunoperoxidase technique to identify nucleated cells in urine. This new development in urinalysis is in its early stages, but it has helped to circumvent the difficulties associated with standard microscopy. The monoclonal antibody technique allowed for the identification of granulocytes, monocytes, lymphocytes, glomerular epithelial, proximal tubular, loop of Henle, distal tubule/collecting duct, and urothelial cells in urine, and by quantifying these cells it was possible to determine the urine cell profiles in various renal diseases as well as in allograft rejection and early post-transplant acute tubular necrosis in renal allograft recipients. The cell profiles are useful in aiding the diagnosis of these conditions.
采用传统的显微镜尿液分析方法准确识别尿液中的有核细胞可能会有困难。单克隆抗体与免疫过氧化物酶技术一起用于识别尿液中的有核细胞。尿液分析的这一新技术尚处于早期阶段，但它有助于克服与标准显微镜检查相关的困难。单克隆抗体技术能够识别尿液中的粒细胞、单核细胞、淋巴细胞、肾小球上皮细胞、近端肾小管细胞、髓袢、远端小管/集合管细胞以及尿路上皮细胞，通过对这些细胞进行定量分析，可以确定各种肾脏疾病、同种异体移植排斥反应以及肾移植受者移植后早期急性肾小管坏死中的尿液细胞谱。这些细胞谱有助于辅助诊断这些病症。
