Sjögren A M, Holme T, Risling M
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Diagn Microbiol Infect Dis. 1988 May;10(1):7-21. doi: 10.1016/0732-8893(88)90122-8.
Cross-reactions between alpha-streptococci and the pneumococcal C-polysaccharide (PnC) were investigated using electron microscopy and immunogold labelling of bacterial cells. Monoclonal antibodies against two different determinants of the PnC molecule were used, one directed against the chain sugar of the repeating unit 2-acetamido-4-amino-2,4,6-trideoxygalactose (Sug) and the other against the phosphorylcholine residue. Two different immunogold techniques were tested, either by direct labelling of the monoclonal antibody or by using an antimouse immunogold conjugate to demonstrate binding of the monoclonal antibodies. Antibodies against the "Sug" determinant reacted only with pneumococci, whereas antibodies against the phosphorylcholine determinant bound to cross-reacting streptococci as well as to pneumococci. These results indicate that the cross-reacting antigens of the alpha-streptococci contain phosphorylcholine residues, but that they are not identical to the C-polysaccharide molecule.
利用电子显微镜和细菌细胞免疫金标记技术，研究了α-链球菌与肺炎球菌C多糖（PnC）之间的交叉反应。使用了针对PnC分子两个不同决定簇的单克隆抗体，一种针对重复单元2-乙酰氨基-4-氨基-2,4,6-三脱氧半乳糖（Sug）的链糖，另一种针对磷酰胆碱残基。测试了两种不同的免疫金技术，一种是直接标记单克隆抗体，另一种是使用抗小鼠免疫金缀合物来证明单克隆抗体的结合。针对“Sug”决定簇的抗体仅与肺炎球菌反应，而针对磷酰胆碱决定簇的抗体则与交叉反应性链球菌以及肺炎球菌结合。这些结果表明，α-链球菌的交叉反应性抗原含有磷酰胆碱残基，但它们与C多糖分子并不相同。
