Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.
Pathology Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione G. Pascale, IRCCS, Naples, 80131, Italy.
J Oral Pathol Med. 2019 Feb;48(2):108-114. doi: 10.1111/jop.12808. Epub 2018 Dec 16.
ADAR1 is an enzymatic protein, which catalyzes a RNA editing reaction by converting Adenosine to Inosine, and its expression has been found to be dysregulated in many cancer types. The aim of this study was to analyze the expression of ADAR1 in oral squamous cells carcinoma.
In order to analyze the ADAR1 mRNA expression, data from The Cancer Genome Atlas (TCGA) database were downloaded and analyzed. In addition, immunohistochemistry analysis was performed on an institutional database including 46 samples of oral squamous cell carcinoma in a tissue microarray (TMA).
No statistically significant correlation linked the mRNA ADAR1 expression to any clinic-pathological variables in the TCGA database. Immunohistochemistry analysis of ADAR1 showed different expressions between normal mucosa and tumor tissue. Focusing on the subcellular localization, the nuclear expression of ADAR1 correlated with higher grading of differentiation (ρ = 0.442; P-value = 0.002); the general expression of ADAR1 either in cytoplasm or in nuclei, correlated with the Gender of patients (Cytoplasm expression: ρ = -0.295; P-value = 0.049; while for nuclear expression: ρ = +0.374; P = 0.011); cytosol expression resulted to be an independent protective prognostic factor (HR = 0.047; C.I. 95% 0.007-0.321; P-value = 0.002).
Higher expression of ADAR1 into the cytoplasm resulted to be an independent prognostic factor. In order to understand ADAR1 role in cancer, further studies should be performed, in bigger cohort and under a bio-molecular point of view.
ADAR1 是一种酶蛋白，通过将腺苷转化为肌苷来催化 RNA 编辑反应，其表达在许多癌症类型中被发现失调。本研究旨在分析 ADAR1 在口腔鳞状细胞癌中的表达。
为了分析 ADAR1 mRNA 的表达，从癌症基因组图谱（TCGA）数据库下载并分析数据。此外，在包含 46 例口腔鳞状细胞癌组织微阵列（TMA）的机构数据库中进行了免疫组织化学分析。
TCGA 数据库中 ADAR1 mRNA 的表达与任何临床病理变量之间无统计学显著相关性。ADAR1 的免疫组化分析显示正常黏膜和肿瘤组织之间的表达不同。聚焦于亚细胞定位，ADAR1 的核表达与较高的分化分级相关（ρ=0.442；P 值=0.002）；细胞质或核中 ADAR1 的一般表达与患者的性别相关（细胞质表达：ρ=-0.295；P 值=0.049；而核表达：ρ=0.374；P=0.011）；细胞质表达是独立的保护预后因素（HR=0.047；95%CI 0.007-0.321；P 值=0.002）。
细胞质中 ADAR1 的高表达是独立的预后因素。为了了解 ADAR1 在癌症中的作用，应进一步在更大的队列和生物分子角度进行研究。
