与基因dcm相关的极短片段错配修复活性并非由编码EcoRII甲基化酶的质粒赋予。
Very short patch mismatch repair activity associated with gene dcm is not conferred by a plasmid coding for EcoRII methylase.
作者信息
Lieb M, Bhagwat A S
机构信息
Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
出版信息
J Bacteriol. 1988 Oct;170(10):4967-8. doi: 10.1128/jb.170.10.4967-4968.1988.
Abstract
The only cytosine methylase in Escherichia coli K-12 methylates the second cytosine in the sequence CC (A/T)GG and is encoded by gene dcm. Methylation and very short patch mismatch repair activities lacking in a dcm mutant of E. coli were restored by a plasmid containing the cloned dcm gene. In contrast, plasmids with the gene for EcoRII methylase, which is a homolog of dcm, restored only cytosine methylase activity and not mismatch repair.
摘要
大肠杆菌K-12中唯一的胞嘧啶甲基化酶可使序列CC（A/T）GG中的第二个胞嘧啶发生甲基化，该酶由dcm基因编码。含有克隆dcm基因的质粒可恢复大肠杆菌dcm突变体中缺乏的甲基化和极短片段错配修复活性。相比之下，携带与dcm同源的EcoRII甲基化酶基因的质粒只能恢复胞嘧啶甲基化酶活性，而不能恢复错配修复活性。
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本文引用的文献
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