Mycology Unit, Medical School and IISPV, University Rovira i Virgili, C/ Sant Llorenç 21, 43201 Reus, Spain.
Unidad de Microbiología, Departamento de Tecnología Médica, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Chile.
Med Mycol. 2019 Aug 1;57(6):733-738. doi: 10.1093/mmy/myy132.
Although there have been few reports of opportunistic infections (superficial and systemic) caused by coelomycetous fungi, they are becoming more frequent. Neocucurbitaria keratinophila (formerly Pyrenochaeta keratinophila), characterized by producing pycnidial conidiomata and small hyaline conidia, seems to be an emergent opportunistic pathogen in Spain. Since this fungus was first reported from human keratitis, eight strains have been isolates from clinical cases in Spain. This is a retrospective study of these fungal strains, including phenotypic and molecular characterizations, and in vitro antifungal susceptibility assays. These clinical strains were identified by sequencing four phylogenetic markers such as the internal transcribed spacer region (ITS1-5.8S-ITS2) and fragments of the 28S nrRNA (LSU), beta-tubulin (tub2), and RNA polymerase II subunit 2 (rpb2) genes, and by morphological characterization. All the strains tested were susceptible to the majority of antifungals, being isavuconazole the only drug that showed a poor antifungal activity.
虽然由腔菌引起的机会性感染（浅表性和系统性）的报道很少，但它们变得越来越频繁。Neocucurbitaria keratinophila（以前称为Pyrenochaeta keratinophila），其特征是产生厚壁孢子的产孢结构和小的透明分生孢子，似乎是西班牙的一种新出现的机会性病原体。自从这种真菌首次从人类角膜炎中报告以来，已经从西班牙的临床病例中分离出了 8 株。这是对这些真菌菌株的回顾性研究，包括表型和分子特征以及体外抗真菌药敏试验。这些临床菌株通过测序四个系统发育标记物（如内部转录间隔区（ITS1-5.8S-ITS2）和 28S nrRNA（LSU）、β-微管蛋白（tub2）和 RNA 聚合酶 II 亚基 2（rpb2）基因的片段）和形态特征进行鉴定。所有测试的菌株对大多数抗真菌药物均敏感，伊曲康唑是唯一显示出较差抗真菌活性的药物。
