Ahmed Sarah A, Stevens David A, van de Sande Wendy W J, Meis Jacques F, de Hoog G S
Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, The Netherlands.
California Institute for Medical Research, San Jose, and Division of Infectious Diseases, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA
Med Mycol. 2014 Oct;52(7):689-98. doi: 10.1093/mmy/myu035. Epub 2014 Jun 25.
We report the isolation of a novel agent of subcutaneous mycosis from a 45-year-old Indian male immigrant in the United States. Phylogenetic analysis of partial small ribosomal subunit and large ribosomal subunit, internal transcribed spacer, partial translation elongation factor (TEF1), and RNA polymerase second largest subunit (rPB2) loci revealed that the strain was identical to another isolate previously reported as "Madurella mycetomatis." Both strains clustered in the order Pleosporales, nested in the family Arthopyreniaceae/Roussoellaceae. The fungus differed from known species and hence a new taxon, Roussoella percutanea, is introduced, typified by a strain that showed delayed production of pycnidial conidiomata. Antifungal susceptibility testing suggested that the new species is resistant to echinocandins and flucytosine, with variable results with azoles and amphotericin B.
我们报告了从一名45岁在美国的印度男性移民身上分离出一种新型皮下真菌病病原体。对部分小核糖体亚基和大核糖体亚基、内转录间隔区、部分翻译延伸因子（TEF1）以及RNA聚合酶第二大亚基（rPB2）基因座进行系统发育分析发现，该菌株与先前报道为“马杜拉分支菌”的另一种分离株相同。这两种菌株都聚集在格孢腔菌目，隶属于节皮盘菌科/鲁氏盘菌科。该真菌与已知物种不同，因此引入了一个新的分类单元——皮肤鲁氏盘菌，其模式菌株表现出分生孢子器产孢延迟。抗真菌药敏试验表明，该新物种对棘白菌素和氟胞嘧啶耐药，对唑类药物和两性霉素B的结果不一。
