Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China.
Bioresour Technol. 2019 Feb;274:1-8. doi: 10.1016/j.biortech.2018.11.070. Epub 2018 Nov 20.
To address the issue of insufficient electrons during denitrification, an iron fuel cell (IFC) bioreactor using iron as abiotic anode was designed. The nitrogen removal efficiency (NRE) of IFC (2.54 ± 0.016%) was significantly lower than microbial fuel cell (MFC) (32.58 ± 0.033%) with same bio-cathode under autotrophic conditions, which was due to the permeation of acetate on proton exchange membrane (PEM) affected the process of enriching autotrophic denitrifying bacteria by MFC. When used in heterotrophic conditions, the NRE of the closed-circuits of IFC was 29.04%, 10.53%, 8.33% higher than open-circuits, respectively, when the COD/nitrogen (C/N) ratios was 1, 2 and 3. The enhancement of IFC was the iron anode could convert a portion of nitrate to nitrite according to the abiotic cathode control experiments. The mainly functional bacteria of bio-cathode was Paracoccus (53.04%). In conclusion, the IFC could be a theoretical model for using inorganic electron donor during denitrification.
为了解决反硝化过程中电子不足的问题，设计了一种以铁为非生物阳极的铁燃料电池（IFC）生物反应器。在自养条件下，IFC（2.54±0.016%）的氮去除效率（NRE）明显低于具有相同生物阴极的微生物燃料电池（MFC）（32.58±0.033%），这是由于乙酸盐通过质子交换膜（PEM）的渗透影响了 MFC 富集自养反硝化细菌的过程。在异养条件下，当 COD/氮（C/N）比分别为 1、2 和 3 时，IFC 的闭路 NRE 分别比开路高 29.04%、10.53%和 8.33%。IFC 的增强是由于铁阳极可以根据非生物阴极控制实验将一部分硝酸盐转化为亚硝酸盐。生物阴极的主要功能细菌是 Paracoccus（53.04%）。总之，IFC 可以作为反硝化过程中使用无机电子供体的理论模型。
