Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province, Chinese Academy of Sciences, Nanjing 210014, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Botany, Jiangsu Province, Chinese Academy of Sciences, Nanjing 210014, China.
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province, Chinese Academy of Sciences, Nanjing 210014, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Botany, Jiangsu Province, Chinese Academy of Sciences, Nanjing 210014, China; College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Pestic Biochem Physiol. 2018 Nov;152:114-121. doi: 10.1016/j.pestbp.2018.09.008. Epub 2018 Sep 20.
10-deacetyl-bacatin III (10-DAB) is a natural plant-derived taxane diterpene, whose antimicrobial activity against phytopathogens remains unknown. In this study, we demonstrated the antimicrobial effect of 10-DAB on plant-pathogenic oomycetes. Our results revealed that 10-DAB exhibited significant antimicrobial activities against test oomycetes, especially against Phytophthora capsici, with a median effective concentration (EC) of 1.46 μg/mL, but had no effect on test fungi. Under 10-DAB treatment, mycelia of P. capsici were contorted with an increased number of top branches, and the production and germination of zoospores were inhibited and delayed, respectively. In addition, 10-DAB had favorable protective and curative activities with control efficacies of 63.90% and 74.81% at 200 μg/mL on detached pepper leaves. Furthermore, 10-DAB caused a significant decrease in soluble protein, lysine, and α, ε-diaminopimelic acid content of P. capsici, which suggested that 10-DAB inhibited the lysine biosynthesis. On the contrary, treatment with exogenous lysine effectively counteracted 10-DAB's inhibition activity on P. capsici. Moreover, relative expression of four key lysine biosynthesis-related genes of P. capsici were decreased upon 10-DAB treatment. Taken together, our findings suggest a lysine biosynthesis inhibiting-dependent antimicrobial activity of 10-DAB against P. capsici, which contributes to accelerating the application of 10-DAB for successful management of phytophthora blight disease in agricultural production.
10-去乙酰巴卡丁 III（10-DAB）是一种天然植物来源的紫杉二萜，其对植物病原菌的抗菌活性尚不清楚。在本研究中，我们证明了 10-DAB 对植物病原卵菌的抗菌作用。我们的结果表明，10-DAB 对测试卵菌表现出显著的抗菌活性，特别是对辣椒疫霉，其半数有效浓度（EC）为 1.46μg/mL，但对测试真菌没有影响。在 10-DAB 处理下，辣椒疫霉菌丝扭曲，顶枝数量增加，游动孢子的产生和萌发分别受到抑制和延迟。此外，10-DAB 在离体辣椒叶片上以 200μg/mL 的浓度具有良好的保护和治疗作用，防治效果分别为 63.90%和 74.81%。此外，10-DAB 导致辣椒疫霉菌可溶性蛋白、赖氨酸和α、ε-二氨基庚二酸含量显著下降，表明 10-DAB 抑制了赖氨酸的生物合成。相反，外源赖氨酸处理有效拮抗了 10-DAB 对辣椒疫霉菌的抑制活性。此外，10-DAB 处理后辣椒疫霉菌四个关键赖氨酸生物合成相关基因的相对表达量降低。综上所述，我们的研究结果表明，10-DAB 对辣椒疫霉具有赖氨酸生物合成抑制依赖性的抗菌活性，这有助于加速 10-DAB 在农业生产中成功防治疫霉病的应用。
