Garg Neha, Kotru Mrinalini, Kumar Dilip, Pathak Rajesh, Sikka Meera
Departments of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India.
Department of Pathology, Max Hospital Saket, New Delhi, India.
J Lab Physicians. 2018 Oct-Dec;10(4):410-413. doi: 10.4103/JLP.JLP_35_18.
Aberrant expression of immunophenotypic markers is commonly found in patients of acute leukemia. T-ALL also shows aberrant markers such as CD13, CD33, CD117, CD10, and CD79a. Morphologically, T-ALL has been categorized into L1, L2, and L3 subtypes. Till now, no study has been done to correlate these markers with morphological features of T-ALL. This study aimed to correlate the expression of aberrant immunophenotypic markers with morphology in T-ALL.
All the cases of T-ALL diagnosed by flow cytometry over a period of 2½ year were taken out from the records of Hematology Section of Department of Pathology of University College of Medical Science and Guru Teg Bahadur Hospital and Max Hospital, Saket. Their peripheral blood smear was screened to correlate the morphology of blasts with the expression of aberrant markers.
A total of 40 cases of T-ALL were identified during 2½ year period of our study. Morphological correlation was available for 23 cases. Aberrant expression of CD10 was present in 6 (35.3%) cases, CD79a in 9 (47.36%) cases, CD117 in 5 (42.28%) cases and myeloid antigen CD33 in 5 (38.46%) cases. CD117 and CD33-positive cases showed L2 morphology with the presence of convolutions, while cases with expression of CD79a had L1 morphology with absent-slight convolutions. CD10-positive cases had L1/L2 morphology with absent occasionally present convolutions.
There seems to be an association of aberrant markers with L1 and L2 morphology. However, this needs to be tested for statistical significance on a larger sample size.
免疫表型标志物的异常表达在急性白血病患者中普遍存在。T 细胞急性淋巴细胞白血病（T-ALL）也表现出异常标志物，如 CD13、CD33、CD117、CD10 和 CD79a。在形态学上，T-ALL 已被分为 L1、L2 和 L3 亚型。到目前为止，尚未有研究将这些标志物与 T-ALL 的形态学特征相关联。本研究旨在将异常免疫表型标志物的表达与 T-ALL 的形态学相关联。
从医学科学大学学院病理系血液科以及古鲁·特格·巴哈杜尔医院和萨克特马克斯医院的记录中取出在 2 年半时间内通过流式细胞术诊断的所有 T-ALL 病例。对其外周血涂片进行筛查，以将原始细胞的形态与异常标志物的表达相关联。
在我们研究的 2 年半期间共鉴定出 40 例 T-ALL。有 23 例可进行形态学关联。CD10 的异常表达存在于 6 例（35.3%）中，CD79a 在 9 例（47.36%）中，CD117 在 5 例（42.28%）中，髓系抗原 CD33 在 5 例（38.46%）中。CD117 和 CD33 阳性病例表现为 L2 形态，有核卷曲，而 CD79a 表达阳性的病例为 L1 形态，无或仅有轻微核卷曲。CD10 阳性病例为 L1/L2 形态，偶尔有核卷曲。
异常标志物似乎与 L1 和 L2 形态有关联。然而，这需要在更大样本量上进行统计学意义的检验。
