Department of Surgery, Centre de recherche du CHU Sainte-Justine, 3175 Côte-Sainte Catherine, Montreal, H3T 1C5, QC, Canada.
University of Montreal, Montreal, QC, Canada.
Eur Spine J. 2019 Jan;28(1):114-120. doi: 10.1007/s00586-018-5818-3. Epub 2018 Nov 29.
To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development.
A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and eighth left ribs. Two days after birth, respiratory system mechanics was assessed in anesthetized lambs using the flexiVent apparatus, followed by postmortem measurement of lung mechanics as well as histological lung analysis.
A range of severity of CWD was found (Cobb angle from 0° to 48°) with a mean decrease in compliance of 47% and in inspiratory capacity of 39% compared to control lambs. Proof-of-concept histological analysis in one lamb showed marked lung hypoplasia.
Our ovine model represents a pilot proof-of-concept study evaluating the impact of a spine and CWD present at birth on lung respiratory mechanics and development. This study lays down the groundwork for future studies evaluating the impact of these deformities on lung development and potential treatments. These slides can be retrieved under Electronic Supplementary Material.
建立一种出生时脊柱和胸廓畸形（CWD）的动物模型，并评估其对呼吸系统力学和肺发育的影响。
在 6 只绵羊胎儿妊娠 70-75 天时，通过切除第 7 和第 8 对左侧肋骨，在子宫内创建脊柱和 CWD。出生后 2 天，使用 flexiVent 仪器对麻醉羔羊进行呼吸系统力学评估，然后进行肺力学的死后测量以及组织学肺分析。
发现 CWD 的严重程度范围很广（Cobb 角为 0°至 48°），与对照组羔羊相比，顺应性降低了 47%，吸气量降低了 39%。对一只羔羊进行的概念验证组织学分析显示，肺部明显发育不良。
我们的绵羊模型代表了一项初步的概念验证研究，评估了出生时存在的脊柱和 CWD 对肺呼吸力学和发育的影响。这项研究为未来评估这些畸形对肺发育和潜在治疗的影响的研究奠定了基础。这些幻灯片可在电子补充材料中检索。
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