Shen Jesse, Samson Nathalie, Lamontagne-Proulx Jérôme, Soulet Denis, Tremblay Yves, Bazin Marc, Nadeau Charlène, Bouchard Sarah, Praud Jean-Paul, Parent Stefan
Centre de recherche du CHU Sainte-Justine Department of Surgery Montreal Quebec Canada.
University of Montreal Department of Surgery Montreal Quebec Canada.
JOR Spine. 2023 Oct 18;7(1):e1295. doi: 10.1002/jsp2.1295. eCollection 2024 Mar.
The evolution and treatment of lung alterations related to congenital spine and chest wall deformities (CWD) are poorly understood. Most animal models of CWD created postnatally were not evaluated for respiratory function. The goal of our study was to evaluate the effects of a CWD induced in utero on lung growth and function in an ovine model.
A CWD was induced in utero at 70-75 days of gestation in 14 ovine fetuses by resection of the 7th and 8th left ribs. Each non-operated twin fetus was taken as control. Respiratory mechanics was studied postnatally in the first week and at 1, 2, and 3 months. Post-mortem respiratory mechanics and lung histomorphometry were also assessed at 3 months.
Eight out of 14 CWD lambs (57%) and 14 control lambs survived the postnatal period. One severe and five mild deformities were induced. At birth, inspiratory capacity (25 vs. 32 mL/kg in controls), and dynamic (1.4 vs. 1.8 mL/cmHO/kg), and static (2.0 vs. 2.5 mL/cmHO/kg) respiratory system compliances were decreased in CWD lambs. Apart from a slight decrease in inspiratory capacity at 1 month of life, no other differences were observed in respiratory mechanics measured in vivo thereafter. Postmortem measurements found a significant decrease in lung compliance-for each lung and for both lungs taken together-in CWD lambs. No differences in lung histology were detected at 3 months in CWD animals compared to controls.
Our study is the first to assess the effects of a prenatally induced CWD on lung development and function from birth to 3 months in an ovine model. Our results show no significant differences in lung histomorphometry at 3 months in CWD lambs compared to controls. Resolution at 1 month of the alterations in respiratory mechanics present at birth may be related to the challenge in inducing severe deformities.
与先天性脊柱和胸壁畸形（CWD）相关的肺部改变的演变及治疗仍知之甚少。大多数出生后创建的CWD动物模型未对呼吸功能进行评估。我们研究的目的是评估子宫内诱导的CWD对绵羊模型肺生长和功能的影响。
在妊娠70 - 75天时，通过切除14只绵羊胎儿的左第7和第8肋骨在子宫内诱导CWD。每只未手术的双胎胎儿作为对照。在出生后第一周以及1、2和3个月时研究呼吸力学。在3个月时还评估了死后呼吸力学和肺组织形态学。
14只CWD羔羊中有8只（57%）和14只对照羔羊存活至出生后。诱导出1例严重畸形和5例轻度畸形。出生时，CWD羔羊的吸气容量（对照组为32 mL/kg，CWD组为25 mL/kg）以及动态（对照组为1.8 mL/cmH₂O/kg，CWD组为1.4 mL/cmH₂O/kg）和静态（对照组为2.5 mL/cmH₂O/kg，CWD组为2.0 mL/cmH₂O/kg）呼吸系统顺应性降低。除了在1月龄时吸气容量略有下降外，此后在体内测量的呼吸力学未观察到其他差异。死后测量发现CWD羔羊的每侧肺以及两侧肺的总肺顺应性均显著降低。与对照组相比，3个月时CWD动物的肺组织学未检测到差异。
我们的研究首次在绵羊模型中评估了产前诱导的CWD从出生到3个月对肺发育和功能的影响。我们的结果表明，与对照组相比，3个月时CWD羔羊的肺组织形态学无显著差异。出生时存在的呼吸力学改变在1个月时得到缓解，这可能与诱导严重畸形的难度有关。
