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Structure of helical RecA-DNA complexes. III. The structural polarity of RecA filaments and functional polarity in the RecA-mediated strand exchange reaction.

作者信息

Stasiak A, Egelman E H, Howard-Flanders P

机构信息

Institute for Cell Biology, E.T.H. Honggerberg, Zurich, Switzerland.

出版信息

J Mol Biol. 1988 Aug 5;202(3):659-62. doi: 10.1016/0022-2836(88)90293-8.

DOI:10.1016/0022-2836(88)90293-8
PMID:3050129
Abstract

The RecA protein of Escherichia coli has been used in vitro to mediate a strand-exchange reaction between homologous DNA molecules. A three-dimensional reconstruction of a RecA filament on double-stranded DNA has been previously determined from electron micrographs, and the reconstruction displays a clear axial polarity. The RecA-mediated strand-exchange reaction between a double-stranded DNA and a homologous single-stranded DNA that is complexed with a RecA helical polymer proceeds with a known polarity. Using image analysis of electron micrographs, we have determined the relation between the structural polarity of RecA filaments and the 3' and 5' polarity of single-stranded DNA. Thus, the structural polarity of RecA filaments can now be related to the direction in which the RecA-mediated strand-exchange reaction advances along the complexed single-stranded DNA.

摘要

相似文献

1
Structure of helical RecA-DNA complexes. III. The structural polarity of RecA filaments and functional polarity in the RecA-mediated strand exchange reaction.
J Mol Biol. 1988 Aug 5;202(3):659-62. doi: 10.1016/0022-2836(88)90293-8.
2
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Base pair switching by interconversion of sugar puckers in DNA extended by proteins of RecA-family: a model for homology search in homologous genetic recombination.通过RecA家族蛋白延伸的DNA中糖环构象互变实现的碱基对切换：同源基因重组中同源性搜索的模型
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J Mol Biol. 1988 Mar 20;200(2):329-49. doi: 10.1016/0022-2836(88)90245-8.

引用本文的文献

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3
A 5'-to-3' strand exchange polarity is intrinsic to RecA nucleoprotein filaments in the absence of ATP hydrolysis.
在没有 ATP 水解的情况下，RecA 核蛋白丝中的 5'到 3'链交换极性是固有性质。
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Caught in the act: the lifetime of synaptic intermediates during the search for homology on DNA.当场被抓：在 DNA 同源性搜索过程中突触中间产物的寿命。
Nucleic Acids Res. 2010 Apr;38(6):2036-43. doi: 10.1093/nar/gkp1177. Epub 2009 Dec 30.
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Organized unidirectional waves of ATP hydrolysis within a RecA filament.RecA丝内ATP水解的有序单向波。
PLoS Biol. 2005 Feb;3(2):e52. doi: 10.1371/journal.pbio.0030052. Epub 2005 Feb 8.
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Topological testing of the mechanism of homology search promoted by RecA protein.RecA蛋白促进的同源性搜索机制的拓扑学测试。
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