[血管内皮生长因子通过Notch信号通路对小鼠胚胎干细胞分化为造血干细胞或造血祖细胞的影响]

[Effect of Notch Signaling Pathway on Differentiation of Mouse Embryonic Stem Cells into Hematopoietic Stem Cells or Hematopoietic Progenitor Cells by VEGF].

作者信息

Song Bo, Chen Ri-Ling, Liao Feng-Ling

机构信息

Department of Pediatrics, Guangdong Medical University,Guangdong Province, China.

Medical center of Pediatrics, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, Guangdong Province, China.E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Dec;26(6):1780-1784. doi: 10.7534/j.issn.1009-2137.2018.06.033.

DOI:10.7534/j.issn.1009-2137.2018.06.033

PMID:30501720

Abstract

OBJECTIVE

To investigate the effect of Notch signaling pathways on differentiation of mouse embryonic stem cells(ESC) into haematopoietic stem cells or haematopoietic progenitors cells(HSC/HPC).

METHODS

Mouse embryonic stem cells were proliferated in vitro to form embryoid bodies; the differentiation of embryoid bodies should be induced in vitro, the experiments were divided into BE, control, VEGF, DAPT and VEGF-DAPT groups; HSC/HPC ohenotype: CD117D34Sca1 was detected by flow cytometry; the related gene expression was detected by RT-PCR.

RESULTS

The number of VEGF-induced HSC/HPC in VEGF group was significantey higher than that in the control and EB group (P<0.05), suggesting that VEGF promotes ESC differentiation to HSC/HPC; the number of DAPT-induced HSC/HPC in DAPT group was significanty higher than that in the Control and EB groups(P<0.05), suggesting that DAPT promotes ESC differentiation to HSC/HPC; the number of VEGF+DAPT-induced HSC/HPC in VEGF-DAPT group was significantly higher than that in VEGF and DAPT groups(P<0.05), suggesting that DAPT and VEGF play a synergistic role to promote differentiation of ESC into HSC/HPC.

CONCLUSION

Notch signal pathway inhibits differentiation of ESC into HSC / HPC by VEGF.

摘要

目的

研究Notch信号通路对小鼠胚胎干细胞（ESC）向造血干细胞或造血祖细胞（HSC/HPC）分化的影响。

方法

体外扩增小鼠胚胎干细胞以形成胚状体；体外诱导胚状体分化，实验分为BE、对照、VEGF、DAPT和VEGF-DAPT组；通过流式细胞术检测HSC/HPC表型：CD117D34Sca1；通过RT-PCR检测相关基因表达。

结果

VEGF组中VEGF诱导的HSC/HPC数量显著高于对照组和EB组（P<0.05），表明VEGF促进ESC向HSC/HPC分化；DAPT组中DAPT诱导的HSC/HPC数量显著高于对照组和EB组（P<0.05），表明DAPT促进ESC向HSC/HPC分化；VEGF-DAPT组中VEGF+DAPT诱导的HSC/HPC数量显著高于VEGF组和DAPT组（P<0.05），表明DAPT和VEGF发挥协同作用促进ESC向HSC/HPC分化。