Using Functionalized Graphene Oxide as Carrier for Immobilization of Glutaryl-7-Aminocephalosporanic Acid Acylase.

Graphene oxide has been widely used in biological applications because of good biocompatibility. In the present work, graphene oxide was synthesized successfully and was employed to immobilize glutaryl-7-aminocephalosporanic acid acylase (GLA). The immobilization conditions like pH, temperature, immobilization time, and enzyme/carrier ratio were investigated. Under the optimal immobilization conditions, the immobilization yield and efficiency were 86% and 52%, respectively. The characterization of the immobilized GLA was performed and compared with the free enzyme. The results indicated that the stability of the immobilized GLA was 1.7-folds than that of the free enzyme, and the kinetic parameters were also calculated. All these results show the potential industrial applications of the immobilized GLA in the production of 7-aminocephalosporinal acid (7-ACA).