基于 RNase H 信号放大的敏感适体荧光分析测定赭曲霉毒素 A。
Sensitive aptamer-based fluorescene assay for ochratoxin A based on RNase H signal amplification.
机构信息
School of Life Sciences, Central South University, Changsha 410013, China.
School of Life Sciences, Central South University, Changsha 410013, China.
出版信息
Food Chem. 2019 Mar 30;277:273-278. doi: 10.1016/j.foodchem.2018.10.130. Epub 2018 Oct 29.
A new assay for the selective and sensitive aptamer-based fluorescent biosensor for ochratoxin A (OTA) detection is proposed. It consists of a ribonuclease H (RNase H)-assisted cycle response that leads to a significant amplification of the signal, which allow for a low limit of detection (LOD) of 0.08 ng/mL. By using this technique, a high selectivity for OTA against ochratoxin B and aflatoxin B (AFB) was noticed. The quantitative determination in real samples has been verified using red wine samples spiked with a series of OTA concentrations (0.4, 4, and 12 ng/mL), and the recoveries ranged from 96.1 to 107.5%. This aptasensor has great practical applications in food industry and moreover, can be extended for the detection of other toxins by replacing the sequence of the recognition aptamer.
提出了一种新的基于适体的选择性和灵敏荧光生物传感器检测赭曲霉毒素 A（OTA）的方法。它由核糖核酸酶 H（RNase H）辅助循环反应组成，导致信号显著放大，从而实现了低检测限（LOD）为 0.08ng/mL。通过使用该技术，对 OTA 对赭曲霉毒素 B 和黄曲霉毒素 B（AFB）具有高选择性。通过用一系列 OTA 浓度（0.4、4 和 12ng/mL）对红酒样品进行加标，验证了实际样品中的定量测定，回收率在 96.1%至 107.5%之间。该适体传感器在食品工业中有很大的实际应用，而且可以通过替换识别适体的序列来扩展用于检测其他毒素。
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