School of Life Sciences, Central South University, Changsha 410013, China.
Toxins (Basel). 2020 Oct 23;12(11):670. doi: 10.3390/toxins12110670.
In this study, we developed an aptamer-based fluorescent sensing platform for the detection of ochratoxin A (OTA) based on RecJ exonuclease-assisted signal amplification and interaction between graphene oxide (GO) and the OTA aptamer (OTA-apt). After optimizing the experimental conditions, the present aptamer-based sensing system can exhibit excellent fluorescent response in the OTA assay, with a limit of detection of 0.07 ng/mL. In addition to signal amplification, this strategy is also highly specific for other interfering toxins. Furthermore, this aptasensor can be reliably used for assessing red wine samples spiked with different OTA concentrations (2.4, 6 and 20 ng/mL). The proposed assay plays an important role in the field of food safety and can be transformed for detecting other toxins by replacing the sequence that recognizes the aptamer.
在这项研究中，我们开发了一种基于适体的荧光传感平台，用于基于 RecJ 外切酶辅助信号放大和氧化石墨烯 (GO) 与赭曲霉毒素 A (OTA) 适体 (OTA-apt) 之间相互作用检测 OTA。在优化实验条件后，本基于适体的传感系统在 OTA 测定中表现出优异的荧光响应，检测限为 0.07ng/mL。除了信号放大外，该策略对其他干扰毒素也具有高度特异性。此外，该适体传感器可用于可靠地评估用不同 OTA 浓度（2.4、6 和 20ng/mL）添加的红酒样品。该测定方法在食品安全领域具有重要作用，通过替换识别适体的序列，可用于检测其他毒素。
