菲律宾河流型水牛（水牛属）感染副结核分枝杆菌亚种（副结核病）的血清学和分子评估
Serological and molecular evaluation of Mycobacterium avium subspecies paratuberculosis (Johne's disease) infecting riverine-type water buffaloes (Bubalus bubalis) in the Philippines.
作者信息
Uy Mary Rose D, Cruz Jeffrey L, Miguel Michelle A, Salinas Marvin Bryan S, Lazaro Jonathan V, Mingala Claro N
机构信息
Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines.
College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines.
出版信息
Comp Immunol Microbiol Infect Dis. 2018 Dec;61:24-29. doi: 10.1016/j.cimid.2018.11.004. Epub 2018 Nov 20.
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease and a possible cause of Crohn's disease in humans. A total of 70 blood and fecal samples were collected from water buffaloes in selected municipalities of Nueva Ecija for ELISA and qPCR assay. Results revealed presence of antibodies of MAP in 3 serum samples for ELISA. The qPCR assay was carried out using standard curve method targeting the MAP specific insertion element IS900. Results revealed that 10 of the samples were positive for MAP DNA in qPCR. ELISA was able to detect antibodies for MAP showing 2.48% infection rate among the 70 buffaloes tested using blood serum samples. On the other hand, qPCR was able to detect MAP using IS900 showed 14.28% infection rate among buffaloes tested using fecal samples. Nucleotide sequence of isolated MAP showed high homology (99-100%) among the reported MAP isolates in the GenBank.
副结核分枝杆菌（MAP）是牛副结核病的病原体，也是人类克罗恩病的一个可能病因。从新怡诗夏省选定市镇的水牛身上总共采集了70份血液和粪便样本，用于酶联免疫吸附测定（ELISA）和定量聚合酶链反应（qPCR）检测。ELISA检测结果显示，3份血清样本中存在MAP抗体。qPCR检测采用针对MAP特异性插入元件IS900的标准曲线法进行。结果表明，10份样本在qPCR检测中MAP DNA呈阳性。ELISA能够检测到MAP抗体，在使用血清样本检测的70头水牛中，感染率为2.48%。另一方面，使用IS900进行的qPCR能够检测到MAP，在使用粪便样本检测的水牛中，感染率为14.28%。分离出的MAP的核苷酸序列与GenBank中报告的MAP分离株具有高度同源性（99-100%）。
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