Bleiberg I, Fabian I, Kantor S, Kletter Y
Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv, Israel.
Leuk Res. 1988;12(7):545-50. doi: 10.1016/0145-2126(88)90083-5.
The modulatory effect of 13-cis retinoic acid (RA) on the growth, differentiation and function of hematopoietic cells in human long-term cultures was studied. RA (5 X 10(-8) M) induced enhancement of myeloid progenitor cell growth in the non-adherent layer throughout 6 weeks of incubation while it did not affect the number of myeloid progenitors in the adherent layer. The vitamin did not alter the differentiation pattern of colony forming unit-culture (CFU-C). The addition of RA to cultures for 5 weeks did not alter the cellular composition of the adherent layer. Prolonged exposure of hematopoietic cells to RA did not affect the functional activity of neutrophils and macrophages, i.e. the cells were active in phagocytosing Candida albicans (CA).
研究了13-顺式维甲酸（RA）对人长期培养中造血细胞生长、分化和功能的调节作用。在整个6周的孵育过程中，RA（5×10⁻⁸M）诱导非贴壁层中髓系祖细胞生长增强，而对贴壁层中髓系祖细胞数量没有影响。该维生素未改变集落形成单位培养（CFU-C）的分化模式。在培养物中添加RA 5周未改变贴壁层的细胞组成。造血细胞长时间暴露于RA并不影响中性粒细胞和巨噬细胞的功能活性，即这些细胞在吞噬白色念珠菌（CA）方面具有活性。
