School of Optical-Electric and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, China; National Pathogen Collection Center for Aquatic Animals, Ministry of Agriculture, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China; College of Mathematics and Computer Science, Chizhou University, China.
Institute of Biomedical Informatics, Lishui University, China; College of Engineering, Lishui University, China.
Biochem Biophys Res Commun. 2019 Jan 8;508(2):374-379. doi: 10.1016/j.bbrc.2018.11.151. Epub 2018 Nov 28.
Colorectal cancer (CRC) is one of the most common malignancies and morbidity and mortality are increasing rapidly. Increasing evidence showed the close correlation between aberrant expression of certain RNAs and the occurrence and development of CRC. However, comprehensive analyses of differentially expressed profiles of linRNA in CRC based on large sample size have been lacking. In the present study, based on RNA-seq data obtained from the TCGA (The Cancer Genome Atlas) database, we identified 1176 lncRNAs, 245 miRNAs and 2083 mRNAs whichaberrantly expressed in the colorectal cancer tissues compared with the adjacent non-tumorous tissues. A Kaplan-Meier curve analysis was used to study the overall survival rate of the three RNA-related CRC patients. After constructing the ceRNA network, we performed the KEGG enrichment pathway analysis on ceRNA-related differentially expressed mRNAs and found that these mRNAs were remarkably enriched in the pathways associated with CRC. Combining the differentially expressed lncRNAs with clinical pathological variables of CRC patients, we also found that LINC00400 and LINC00355 not only contribute to the regulation of ceRNA network, but also show significantchanges in its expression in multiple CRC pathological stages, indicating that LINC00400 and LINC00355 can be considered as promising therapeutic targets for CRC.
结直肠癌（CRC）是最常见的恶性肿瘤之一，发病率和死亡率迅速上升。越来越多的证据表明，某些 RNA 的异常表达与 CRC 的发生和发展密切相关。然而，基于大样本量的 CRC 中 linRNA 差异表达谱的综合分析还很缺乏。在本研究中，我们基于 TCGA（癌症基因组图谱）数据库中的 RNA-seq 数据，鉴定了 1176 个 lncRNA、245 个 miRNA 和 2083 个 mRNAs，这些 RNA 在结直肠癌细胞组织中与相邻的非肿瘤组织相比存在异常表达。我们使用 Kaplan-Meier 曲线分析研究了三种与 RNA 相关的 CRC 患者的总生存率。在构建 ceRNA 网络后，我们对 ceRNA 相关差异表达的 mRNAs 进行了 KEGG 富集途径分析，发现这些 mRNAs显著富集在与 CRC 相关的途径中。将差异表达的 lncRNA 与 CRC 患者的临床病理变量相结合，我们还发现 LINC00400 和 LINC00355 不仅有助于 ceRNA 网络的调控，而且在多个 CRC 病理阶段其表达也发生了显著变化，表明 LINC00400 和 LINC00355 可以被视为 CRC 的有前途的治疗靶点。
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