Mochizuki Kunio, Kawai Masataka, Odate Toru, Tahara Ippei, Inoue Tomohiro, Kasai Kazunari, Nakazawa Tadao, Kondo Tetsuo
Department of Pathology, School of Medicine, University of Yamanashi, Chuo, Japan
Department of Pathology, School of Medicine, University of Yamanashi, Chuo, Japan.
Anticancer Res. 2018 Dec;38(12):6865-6868. doi: 10.21873/anticanres.13061.
BACKGROUND/AIM: Switch/sucrose non-fermentable (SWI/SNF)-related, matrix-associated, actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1), also named integrase interactor 1, is one of the core subunit proteins in the SWI/SNF ATP-dependent chromatin remodeling complex encoded at chromosomal position 22q11.2. Complete loss of SMARCB1 expression has been reported in various malignant tumors. Immunohistochemistry has demonstrated that SMARCB1 mutation/inactivation correlates well with loss of nuclear SMARCB1 expression. This study investigated SMARCB1 expression in hepatocellular carcinomas (HCCs) and α-fetoprotein (AFP)-producing gastric carcinomas by immunohistochemistry. For comparison, SMARCB1 immunostaining in intrahepatic cholangiocarcinoma (ICC) was also performed.
Thirty classical HCCs, 30 ICCs and 10 AFP-producing gastric carcinomas were analyzed.
Only one case of HCC had focal labeling of SMARCB1 in the nuclei. Twelve cases of ICC were immunopositive for SMARCB1, with either focal or diffuse reactivity. All AFP-producing gastric carcinomas were immunopositive for SMARCB1 in the nuclei, and the reactivity was consistently diffuse.
SMARCB1 is a potential marker for distinguishing metastatic AFP-producing gastric carcinoma from HCC.
背景/目的：与开关/蔗糖非发酵相关（SWI/SNF）、基质相关、肌动蛋白依赖的染色质调节因子B亚家族成员1（SMARCB1），也称为整合酶相互作用因子1，是位于染色体22q11.2编码的SWI/SNF ATP依赖染色质重塑复合体中的核心亚基蛋白之一。在各种恶性肿瘤中均有报道SMARCB1表达完全缺失。免疫组织化学表明，SMARCB1突变/失活与核SMARCB1表达缺失密切相关。本研究通过免疫组织化学研究了SMARCB1在肝细胞癌（HCC）和产甲胎蛋白（AFP）的胃癌中的表达。为作比较，还对肝内胆管癌（ICC）进行了SMARCB1免疫染色。
分析了30例经典HCC、30例ICC和10例产AFP的胃癌。
仅1例HCC细胞核有SMARCB1局灶性标记。12例ICC对SMARCB1呈免疫阳性，反应性为局灶性或弥漫性。所有产AFP的胃癌细胞核对SMARCB1均呈免疫阳性，且反应性始终为弥漫性。
SMARCB1是区分转移性产AFP胃癌与HCC的潜在标志物
