高效无痕的人类多能干细胞基因组编辑。
Efficient scarless genome editing in human pluripotent stem cells.
机构信息
Department of Pediatrics, Stanford University, Stanford, CA, USA.
ReGen Med Division, BOCO Silicon Valley, Palo Alto, CA, USA.
出版信息
Nat Methods. 2018 Dec;15(12):1045-1047. doi: 10.1038/s41592-018-0212-y. Epub 2018 Nov 30.
PMID:30504872
Abstract
Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.
摘要
无痕基因组编辑在人类多能干细胞（hPSCs）中代表了一个精确研究应用和 hPSC 衍生疗法临床转化的目标。在这里，我们建立了一种通用且高效的方法，该方法将 CRISPR-Cas9 介导的同源重组与编辑克隆的正负选择相结合，在 hPSCs 中产生无痕的遗传变化。
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