Ikeda Kazuya
Bayspair Inc., 319 Bernardo Avenue, Mountain View, CA 94043, USA.
Breed Sci. 2024 Mar;74(1):32-36. doi: 10.1270/jsbbs.23045. Epub 2024 Mar 9.
The advent of CRISPR/Cas9 has had a disruptive impact on the world by bringing about dramatic progress and rapid penetration of genome editing technology. However, even though gene disruption can be easily achieved, there has been a challenge in freely changing the sequence. To solve this problem, various novel technologies have emerged in recent years to realize free rewriting of genome sequences. In this review, scarless editing by two-step HDR, a technology that can freely rewrite genomes from a single nucleotide to more than several thousand nucleotides, will be introduced.
CRISPR/Cas9的出现给世界带来了颠覆性影响，实现了基因组编辑技术的巨大进步和迅速普及。然而，尽管基因破坏很容易实现，但在自由改变序列方面仍存在挑战。为了解决这个问题，近年来出现了各种新技术以实现基因组序列的自由改写。在这篇综述中，将介绍通过两步同源重组介导的无疤痕编辑技术，该技术能够从单个核苷酸到数千个以上核苷酸自由改写基因组。
