Dept. of Food Technology, Technological Educational Institute of Peloponnese, 24100, Kalamata, Greece.
Biotechnol Prog. 2019 Mar;35(2):e2759. doi: 10.1002/btpr.2759. Epub 2018 Dec 24.
Despite the fact that Saccharomyces cerevisiae has suicide tendencies since its product affects cell function, it is a key player in alcoholic fermentation. The presence of ethanol in the medium affects membrane integrity and fluidity, as well as the rate of ethanol production. The Msn2/4p transcription factors are key regulators in stress response and play a critical role in cell response to ethanol challenge. Protein kinase A (tpk1/2/3) is controlling the activation/inactivation of a multitude of proteins through phosphorylation at specific serine residues. Targets of Protein Kinase A (PKA) are also msn2/4 and phosphorylation of these two transcription factors by PKA resulting in obstruction of their translocation to the nucleus. This work attempts to reveal the significance of specific serine residues of Msn2/4p, as possible targets of PKA, through substitution of these serine residues with alanine. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2759, 2019.
尽管酿酒酵母（Saccharomyces cerevisiae）具有自杀倾向，因为其产物会影响细胞功能，但它却是酒精发酵的关键参与者。培养基中乙醇的存在会影响膜的完整性和流动性，以及乙醇的生产速度。Msn2/4p 转录因子是应激反应的关键调节剂，在细胞对乙醇挑战的反应中起着至关重要的作用。蛋白激酶 A（tpk1/2/3）通过在特定丝氨酸残基上的磷酸化来控制多种蛋白质的激活/失活。蛋白激酶 A（PKA）的靶标也是 msn2/4，PKA 对这两个转录因子的磷酸化导致它们向核内易位受阻。这项工作试图通过用丙氨酸取代这些丝氨酸残基，揭示 Msn2/4p 特定丝氨酸残基作为 PKA 可能靶点的重要性。2018 年美国化学工程师协会生物技术进展，35：e2759，2019。
