Department of Fisheries Science, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran.
School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL.
J Anim Sci. 2018 Dec 3;96(12):5253-5264. doi: 10.1093/jas/sky348.
As global aquaculture continues to expand, increasing efforts are focusing on assisted reproductive technologies. This study sought to test whether salmon GnRH [D-Arg6, Pro9NEt]) analogue (sGnRHa) + domperidone injection at 0.25 mL/body weight (BW; 5-μg sGnRHa + 2.5-mg domperidone), 0.5 mL/kg BW (10-μg sGnRHa + 5-mg domperidone), or 1.0 mL/kg BW (20-μg sGnRHa + 10-mg domperidone) affects spawning performance and gamete quality in wild-caught Levantine scraper, Capoeta damascina. The ability of these treatments to elicit a response was further examined by in vivo stimulation of estradiol (E2) and 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and by its in vivo potency to induce oocyte maturation (OM). Females that received saline injection (control) did not spawn, whereas sGnRHa + domperidone induced ovulation and spawning across the hormonal gradient. Spawning success was highest with the 0.5 mL/kg dosage (80%) and female latency period decreased with increasing dosage. Females treated with 0.5 mL/kg had a significantly higher fecundity than those injected with 0.25 or 1.0 mL/kg. Mean oocyte diameter significantly increased in females treated with 0.5 or 1.0 mL/kg. Fertilization success, hatching rate, larvae morphology, and survival were not affected by hormonal treatment. At 12 h postinjection, E2 levels significantly declined in females treated with 0.5 or 1.0 mL/kg, whereas DHP levels significantly increased across the hormonal gradient. This steroidogenic shift is supported by histological analyses, where OM was accelerated by administration of sGnRHa + domperidone in a dose-dependent manner. In conclusion, the 0.5 mL/kg dosage of sGnRHa + domperidone is recommended for assisted reproduction of Levantine scraper.
随着全球水产养殖业的持续扩张，人们越来越关注辅助生殖技术。本研究旨在测试鲑鱼 GnRH [D-Arg6, Pro9NEt] 类似物（sGnRHa）+ 多潘立酮注射（0.25 毫升/体重（BW）；5-μg sGnRHa + 2.5 毫克多潘立酮）、0.5 毫升/千克 BW（10-μg sGnRHa + 5 毫克多潘立酮）或 1.0 毫升/千克 BW（20-μg sGnRHa + 10 毫克多潘立酮）是否会影响野生捕获的黎凡特刮刀 Capoeta damascina 的繁殖性能和配子质量。通过体内刺激雌二醇（E2）和 17α,20β-二羟基-4-孕烯-3-酮（DHP），并通过其体内诱导卵母细胞成熟（OM）的效力，进一步研究了这些处理方法的反应能力。接受盐水注射（对照）的雌性不会产卵，而 sGnRHa+多潘立酮则在整个激素梯度上诱导排卵和产卵。随着剂量的增加，0.5 毫升/千克剂量的产卵成功率最高（80%），雌性潜伏期缩短。用 0.5 毫升/千克剂量处理的雌性的生育力明显高于用 0.25 或 1.0 毫升/千克剂量处理的雌性。用 0.5 或 1.0 毫升/千克剂量处理的雌性的平均卵直径显著增加。受精成功率、孵化率、幼虫形态和存活率不受激素处理的影响。注射后 12 小时，用 0.5 或 1.0 毫升/千克剂量处理的雌性的 E2 水平显著下降，而 DHP 水平在整个激素梯度上显著增加。这种类固醇生成的转变得到了组织学分析的支持，其中 sGnRHa+多潘立酮以剂量依赖的方式加速 OM。总之，推荐使用 0.5 毫升/千克剂量的 sGnRHa+多潘立酮来辅助繁殖黎凡特刮刀。
