Department of Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Department of Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Biochem Biophys Res Commun. 2019 Jan 8;508(2):516-520. doi: 10.1016/j.bbrc.2018.11.167. Epub 2018 Nov 30.
Cells activate the unfolded protein response (UPR) to cope with endoplasmic reticulum (ER) stress. In the present study, we investigated the possible involvement of psychological stress on UPR induction in the mouse brain. When mice were exposed to immobilization stress for 8 h, XBP1 mRNA splicing was significantly induced in the hippocampus, cortex, hypothalamus, cerebellum, and brain stem. On the other hand, we did not observe any increase in XBP1 splicing in the liver, suggesting that this effect is specific to the brain. Stress-induced XBP1 splicing was attenuated 2 days after immobilization stress. We did not observe increases in any other UPR genes, such as CHOP or GRP78, in mouse brains after immobilization stress. These findings indicate an important specific role of XBP1 in response to psychological stress in the mouse brain.
细胞通过激活未折叠蛋白反应（UPR）来应对内质网（ER）应激。在本研究中，我们研究了心理应激是否可能参与诱导小鼠大脑中的 UPR。当小鼠被束缚应激 8 小时时，XBP1mRNA 的剪接在海马体、皮质、下丘脑、小脑和脑干中明显被诱导。另一方面，我们没有观察到 XBP1 剪接在肝脏中增加，这表明这种效应是大脑特有的。束缚应激 2 天后，应激诱导的 XBP1 剪接减弱。我们没有观察到在束缚应激后，小鼠大脑中任何其他 UPR 基因（如 CHOP 或 GRP78）的增加。这些发现表明 XBP1 在小鼠大脑对心理应激的反应中具有重要的特定作用。
