Castro Rânmilla Cristhina Santos, David de Oliveira Anay Priscilla, Rodrigues de Souza Eline Almeida, Correia Tayla Marielle Antunes, Viana de Souza Jane, Dias Francesca Silva
Federal University of San Francisco Valley, Rod. BR 407, Km 12, Lote 543, Projeto de Irrigação Senador Nilo Coelho, s/nº, C1, BR-56.300-990 Petrolina, Pernambuco, Brazil.
Food Technol Biotechnol. 2018 Sep;56(3):431-440. doi: 10.17113/ftb.56.03.18.5736.
The aim of this study is to investigate the bacterial population in coalho goat cheese produced in the semi-arid northeast region of Brazil, to analyse the antibiotic resistance profiles of the identified pathogenic bacteria, to detect the staphylococcal enterotoxin genes and to evaluate the addition of autochthonous lactic acid bacteria (LAB) with technofunctional properties for the control of growth. In the analysed samples, strains of (=11), spp. (=18), spp. (=6) and (=9) were classified as multidrug resistant (MDR). The most commonly isolated pathogen from the studied coalho goat cheese was . Its isolates were positive for the genes encoding enterotoxins A (), B (), C () and D (). The autochthonous LAB with the potential to inhibit were identified as These strains were selected for tests of protective, safety, technological and functional properties. In the coalho goat cheese food matrix, these selected autochthonous LAB were able to reduce the enterotoxigenic MDR load by approx. 3 log units.
本研究的目的是调查巴西东北部半干旱地区生产的科尔霍山羊奶酪中的细菌菌群，分析已鉴定的病原菌的抗生素耐药性谱，检测葡萄球菌肠毒素基因，并评估添加具有技术功能特性的本地乳酸菌（LAB）对其生长的控制作用。在分析的样本中，（=11）、spp.（=18）、spp.（=6）和（=9）的菌株被归类为多重耐药（MDR）。从研究的科尔霍山羊奶酪中最常分离出的病原体是。其分离株编码肠毒素A（）、B（）、C（）和D（）的基因呈阳性。具有抑制潜力的本地乳酸菌被鉴定为。这些菌株被选用于保护性、安全性、技术和功能特性测试。在科尔霍山羊奶酪食品基质中，这些选定的本地乳酸菌能够将产肠毒素的多重耐药菌负荷降低约3个对数单位。
