Plant Genome. 2018 Nov;11(3). doi: 10.3835/plantgenome2017.11.0097.
Nees & Arn. ex Watt, a perennial wild rice species with a GG genome, preserves many important genes for cultivated rice ( L.) improvement. At present, however, no genetic resource is available for studying . Here, we report 91,562 high-quality transcripts of assembled de novo. Moreover, comparative transcriptome analysis revealed that 1311 single-copy orthologous pairs shared by and (Zoll. & Moritzi) Baill. that may have undergone adaptive evolution. We performed an analysis of the genes potentially involved in plant-pathogen interactions to explore the molecular basis of disease resistance, and isolated the full-length cDNAs of () and () orthologs from . The overexpression of in Nipponbare and functional characterization showed enhanced the resistance of transgenic Nipponbare to rice blast resulting from the presence of the gene. , an alternatively spliced transcript of the blast resistance gene in encodes a 1024-amino acid polypeptide with a C-terminal thioredoxin domain. This study provides an important resource for functional and evolutionary studies of the genus .
Nees & Arn. ex Watt，一种具有 GG 基因组的多年生野生稻种，为栽培稻（L.）的改良保存了许多重要基因。然而，目前尚无遗传资源可用于研究。在这里，我们报告了 91,562 条高质量的从头组装转录本。此外，比较转录组分析显示，和（Zoll. & Moritzi）Baill. 之间有 1311 个单拷贝直系同源对，可能经历了适应性进化。我们对可能参与植物-病原体相互作用的基因进行了分析，以探讨抗病的分子基础，并从中分离出（）和（）直系同源物的全长 cDNA。在中过量表达，并对其功能进行了表征，结果表明，的存在增强了转基因 Nipponbare 对稻瘟病的抗性。是一个抗病基因的可变剪接转录本，编码一个含有 C 端硫氧还蛋白结构域的 1024 个氨基酸多肽。这项研究为该属的功能和进化研究提供了重要资源。
