Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, P. R. China.
Food Funct. 2019 Jan 22;10(1):151-162. doi: 10.1039/c8fo01486h.
Previous studies suggested the anti-diabetic effect of mogrosides in type 1 diabetes. To evaluate the potential effect of mogrosides in type 2 diabetes, we herein investigated the hypoglycemic and hypolipidemic effects and the underlying mechanism of mogroside-rich extract (MGE) using a high-fat diet in combination with streptozotocin (STZ)-induced diabetic model. MGE feeding for 5 weeks did not result in any obvious impact on the body weight and energy intake, but caused a moderate decrease of organ index in diabetic mice. MGE-supplemented diabetic mice showed a notable reduction of fasting blood glucose (FBG), glycated serum protein (GSP), serum insulin, homeostasis model assessment-insulin resistance (HOMA-IR), and serum atherogenic lipid profiles in a dose-dependent manner, whereas significant increases in the anti-atherogenic lipid profile, insulin sensitivity, glucose and insulin tolerance capacity with high dose (300 mg kg-1) MGE were observed (P < 0.01). Besides, hepatocyte polymorphism, lipid accumulation and steatosis were ameliorated and restored to near normal at the high dose. Furthermore, hepatic 5'AMP-activated protein kinase (AMPK) signaling was dose-dependently activated. Accordingly, the mRNA levels of hepatic gluconeogenic and lipogenic genes were downregulated and fat oxidation-associated genes were upregulated. These findings suggest that the hypoglycemic and hypolipidemic activities of MGE are probably attributed to the attenuation of insulin resistance and activation of hepatic AMPK signaling.
先前的研究表明，罗汉果苷在 1 型糖尿病中具有抗糖尿病作用。为了评估罗汉果苷在 2 型糖尿病中的潜在作用，我们在此使用高脂肪饮食联合链脲佐菌素（STZ）诱导的糖尿病模型，研究了富含罗汉果苷的提取物（MGE）的降血糖和降血脂作用及其潜在机制。5 周的 MGE 喂养对糖尿病小鼠的体重和能量摄入没有明显影响，但导致器官指数中度下降。MGE 补充的糖尿病小鼠空腹血糖（FBG）、糖化血清蛋白（GSP）、血清胰岛素、稳态模型评估胰岛素抵抗（HOMA-IR）和血清致动脉粥样硬化脂质谱显著降低，呈剂量依赖性，而高剂量（300mg/kg-1）MGE 可显著增加抗动脉粥样硬化脂质谱、胰岛素敏感性、葡萄糖和胰岛素耐量（P < 0.01）。此外，肝细胞多形性、脂质积累和脂肪变性得到改善，并在高剂量时恢复正常。此外，肝 5'AMP 激活蛋白激酶（AMPK）信号通路呈剂量依赖性激活。相应地，肝糖异生和脂质生成基因的 mRNA 水平下调，脂肪氧化相关基因上调。这些发现表明，MGE 的降血糖和降血脂活性可能归因于胰岛素抵抗的减轻和肝 AMPK 信号的激活。
