Hoare Rowena, Jung Sung-Ju, Ngo Thao P H, Bartie Kerry L, Thompson Kim D, Adams Alexandra
Institute of Aquaculture, University of Stirling, Stirling, UK.
Chonnam National University, Yeosu, Korea.
J Fish Dis. 2019 Feb;42(2):229-236. doi: 10.1111/jfd.12919. Epub 2018 Dec 6.
Flavobacterium psychrophilum is one of the most important pathogens affecting cultured rainbow trout (Oncorhynchus mykiss). Recent information from UK salmonid farms showed country-wide distribution of genetically and serologically divergent clones, which has hampered the development of a vaccine for rainbow trout fry syndrome. The current study assessed the efficacy of an injectable polyvalent vaccine containing formalin-inactivated F. psychrophilum in rainbow trout. The vaccine was formulated with an oil adjuvant (Montanide ISA 760VG) or formalin-killed cells alone. Duplicate groups of trout (60 ± 13 g) were given phosphate-buffered saline or vaccine formulated with Montanide by intra-peritoneal (i.p.) injection and challenged by intra-muscular (i.m.) injection with a homologous and a heterologous isolate of F. psychrophilum at 525 degree days post-vaccination (dd pv). Significant protection was achieved in vaccinated fish (p = 0.0001, RPS 76% homologous, 88% heterologous). Efficacy of the adjuvanted vaccine was also demonstrated by heterologous challenge at 1155 dd pv resulting in 100% protection, whereas survival in the un-adjuvanted group was not significantly different from control fish. Levels of specific antibody at 1155 dd pv, as measured by ELISA, were significantly higher in the fish vaccinated with adjuvant when compared with unvaccinated fish.
嗜冷黄杆菌是影响养殖虹鳟（Oncorhynchus mykiss）的最重要病原体之一。英国鲑鱼养殖场的最新信息显示，在全国范围内存在基因和血清学上不同的克隆体分布，这阻碍了虹鳟鱼苗综合征疫苗的开发。本研究评估了一种含有福尔马林灭活嗜冷黄杆菌的注射用多价疫苗对虹鳟的效力。该疫苗采用油佐剂（Montanide ISA 760VG）或仅用福尔马林灭活的细胞配制。将两组重复的虹鳟（60±13克）通过腹腔注射（i.p.）给予磷酸盐缓冲盐水或含Montanide的疫苗，并在疫苗接种后525度日（dd pv）通过肌肉注射（i.m.）用嗜冷黄杆菌的同源和异源分离株进行攻毒。接种疫苗的鱼获得了显著的保护效果（p = 0.0001，同源RPS为76%，异源RPS为88%）。在1155 dd pv进行异源攻毒时，也证明了佐剂疫苗的效力，其保护率达100%，而未加佐剂组的存活率与对照鱼无显著差异。通过ELISA检测，在1155 dd pv时，接种佐剂疫苗的鱼的特异性抗体水平显著高于未接种疫苗的鱼。
