Josip Juraj Strossmayer University of Osijek, Department of Biology, Ulica cara Hadrijana 8/A, HR-31000, Osijek, Croatia.
Faculty of Agrobiotechnical Sciences, Josip Juraj Strossmayer University of Osijek, Ulica kralja Petra Svačića 1d, HR-31000, Osijek, Croatia.
Aquat Toxicol. 2019 Feb;207:52-62. doi: 10.1016/j.aquatox.2018.11.018. Epub 2018 Nov 27.
This paper aims to evaluate the effects of a product containing colloidal silver in the aquatic environment, using duckweed Lemna gibba as a model plant. Therefore, growth parameters, photosynthetic pigments content and protein content as physiological indices were evaluated. Changes in the content of non-enzymatic antioxidants and activity of several antioxidant enzymes, alongside with the accumulation of hydrogen peroxide and lipid peroxidation end-products were assessed to explore the potential of colloidal silver to induce oxidative stress. The commercially available colloidal silver product contained a primary soluble form of silver. The treatment with colloidal silver resulted in significant physiological and biochemical changes in L. gibba plants and a consequent reduction of growth. Accumulation of silver caused altered nutrient balance in the plants as well as a significant decrease in photosynthetic pigments content and protein concentration. The antioxidative response of L. gibba plants to treatment with colloidal silver was inadequate to protect the plants from oxidative stress caused by metal accumulation. Silver caused concentration-dependent and time-dependent hydrogen peroxide accumulation as well as the elevation of lipid peroxidation levels in L. gibba plants. The use of commercially available products containing colloidal silver, and consequent accumulation of silver, both ionic and nanoparticle form in the environment, represents a potential source of toxicity to primary producers in the aquatic ecosystem.
本文旨在评估含有胶体银的产品在水生环境中的作用，以浮萍（Lemna gibba）作为模式植物。因此，评估了生长参数、光合色素含量和蛋白质含量等生理指标。评估了非酶抗氧化剂含量的变化和几种抗氧化酶的活性，以及过氧化氢和脂质过氧化终产物的积累，以探讨胶体银诱导氧化应激的潜力。市售的胶体银产品含有主要的可溶性银形式。胶体银处理导致浮萍植物发生显著的生理和生化变化，从而导致生长减少。银的积累导致植物中营养平衡的改变以及光合色素含量和蛋白质浓度的显著下降。浮萍植物对胶体银处理的抗氧化反应不足以保护植物免受金属积累引起的氧化应激。银导致过氧化氢的浓度依赖性和时间依赖性积累，并导致浮萍植物中脂质过氧化水平的升高。环境中商业上可获得的含有胶体银的产品的使用，以及随之而来的银的积累，无论是离子形式还是纳米颗粒形式，都代表了对水生生态系统中初级生产者的潜在毒性来源。
