Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, State Key Laboratory Base of Eco-Chemical Engineering, Key Laboratory of Rubber-Plastics of Ministry of Education/Shandong Provincial Key Laboratory of Rubber Plastics, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Department of Pathology, Bao Di Hospital, Bao Di Clinical College of Tianjin Medical University, Tianjin 301800, China.
J Pharm Biomed Anal. 2019 Feb 20;165:94-100. doi: 10.1016/j.jpba.2018.11.059. Epub 2018 Nov 28.
MoS, a typical transition metal disulfide, is widely used in the photoelectrochemical (PEC) sensor construction. In general, MoS based PEC sensor are "signal-on" strategies. Surprisingly, we discovered that the PEC response of MoS was quenched by methionine greatly. Based on this discovery, a reduction PEC sensing strategy utilized MoS modified electrode for methionine detection was fabricated for the first time. Experimental factors, such as, bias potential, volume of MoS and pH were studied. Under optimized conditions, the decreased intensity of the photocurrent signal was proportional to the logarithmic value of methionine concentrations from 0.1 nM to 1 μM with the detection limit of 0.03 nM. Moreover, this method exhibited good performance of excellent selectivity. And it showed potential applications in the practical determination of methionine in real-life sample. This strategy not only expands the PEC detection method but also provides a simple, rapid response, good selectivity and high sensitivity way to detect methionine.
MoS 是一种典型的过渡金属二硫化物，广泛应用于光电化学（PEC）传感器的构建中。一般来说，基于 MoS 的 PEC 传感器采用的是“信号开启”策略。但令人惊讶的是，我们发现甲硫氨酸对 MoS 的 PEC 响应有很大的猝灭作用。基于这一发现，我们首次构建了一种利用 MoS 修饰电极用于甲硫氨酸检测的还原 PEC 传感策略。我们研究了实验因素，如偏置电势、MoS 的用量和 pH 值等。在优化条件下，光电流信号的减小强度与甲硫氨酸浓度的对数成正比，从 0.1 nM 到 1 μM，检测限为 0.03 nM。此外，该方法表现出优异的选择性。并且它在实际生活样品中甲硫氨酸的实际测定中显示出了潜在的应用价值。这种策略不仅扩展了 PEC 检测方法，而且为检测甲硫氨酸提供了一种简单、快速响应、良好选择性和高灵敏度的方法。
